Semi-nested Taqman real-time quantitative PCR for noninvasive prenatal diagnosis of Bart's hydrops fetalis

• Main Authors: Pornprasert S., Sukunthamala K., Kunyanone N., Sittiprasert S., Thungkham K., Junorse S., Pongsawatkul K., Pattanaporn W., Jitwong C.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.ncbi.nlm.nih.gov/pubmed/3502482; http://www.scopus.com/inward/record.url?eid=2-s2.0-84856878134&partnerID=40&md5=2b0016c9d12a6593c9b0607e3f1e54bc; http://cmuir.cmu.ac.th/handle/6653943832/800
• Institution: Chiang Mai University

Background: Non-invasive prenatal diagnosis based on detection of fetal cell-free DNA is limited when mother and father are both carriers for the same autosomal recessive mutation. Objective: Develop the semi-nested Taqman real-time PCR for quantification of α-thalassemia-1 SEA type deletion allele...