A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
Couples in which both partners carry the α-thalassemia-1 trait have a 25% risk of hemoglobin Bart's hydrops fetalis in each pregnancy. Identification of α-thalassemia-1 trait is, therefore, necessary in order to control this severe form of α-thalassemia. We have generated monoclonal antibodies...
محفوظ في:
| المؤلفون الرئيسيون: | , , , , |
|---|---|
| التنسيق: | مقال |
| اللغة: | English |
| منشور في: |
2014
|
| الوصول للمادة أونلاين: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84890324046&partnerID=40&md5=86c8ae6d1a34ca37cada856aee1980f4 http://cmuir.cmu.ac.th/handle/6653943832/933 |
| الوسوم: |
إضافة وسم
لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!
|
| المؤسسة: | Chiang Mai University |
| اللغة: | English |
| الملخص: | Couples in which both partners carry the α-thalassemia-1 trait have a 25% risk of hemoglobin Bart's hydrops fetalis in each pregnancy. Identification of α-thalassemia-1 trait is, therefore, necessary in order to control this severe form of α-thalassemia. We have generated monoclonal antibodies specific to the ζ-globin chain without cross reaction with other globin chains. A simple and sensitive ELISA was developed by using poly-l-lysine to increase the protein binding to the ELISA plate. The developed poly-l-lysine pre-coated ELISA has a very high sensitivity (100%) and specificity (97%) for detection of carriers of α-thalassemia-1 with Southeast Asian-type deletion. © 2014 Copyright Taylor and Francis Group, LLC. |
|---|