Human sex determination by amelogenin padlock probes
In this study, we utilized a technique called ligation-mediated rolling circle amplification (L-RCA) that combined padlock probe ligation and rolling circle amplification techniques to determine human sex. Amelogenin X and Y specific padlock probes were designed based on polymorphisms and the 6-bp i...
Saved in:
Main Authors: | , , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2018
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/11411 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.11411 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.114112018-05-03T15:21:00Z Human sex determination by amelogenin padlock probes Piyarat Thaijaroen Korapin Srisiri Rapee Boonplueang Nathinee Panvisavas Mahidol University Biochemistry, Genetics and Molecular Biology Medicine In this study, we utilized a technique called ligation-mediated rolling circle amplification (L-RCA) that combined padlock probe ligation and rolling circle amplification techniques to determine human sex. Amelogenin X and Y specific padlock probes were designed based on polymorphisms and the 6-bp indel of the amelogenin X and Y alleles. Lengths of DNA target for padlock probe detection were 40 and 33 bases of amelogenin X and Y alleles, respectively. Analysis of female DNA samples produced one L-RCA product from the AmelX padlock probe, whereas 2 different types of products were amplified from male DNA samples, each generated by AmelX and AmelY padlock probes. Utilization of padlock probes via L-RCA technique would provide an alternative tool for human sex determination in highly degraded DNA analysis in the future. © 2011 Elsevier Ireland Ltd. 2018-05-03T07:58:39Z 2018-05-03T07:58:39Z 2011-12-01 Article Forensic Science International: Genetics Supplement Series. Vol.3, No.1 (2011) 10.1016/j.fsigss.2011.09.110 1875175X 18751768 2-s2.0-82455164317 https://repository.li.mahidol.ac.th/handle/123456789/11411 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=82455164317&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Biochemistry, Genetics and Molecular Biology Medicine |
spellingShingle |
Biochemistry, Genetics and Molecular Biology Medicine Piyarat Thaijaroen Korapin Srisiri Rapee Boonplueang Nathinee Panvisavas Human sex determination by amelogenin padlock probes |
description |
In this study, we utilized a technique called ligation-mediated rolling circle amplification (L-RCA) that combined padlock probe ligation and rolling circle amplification techniques to determine human sex. Amelogenin X and Y specific padlock probes were designed based on polymorphisms and the 6-bp indel of the amelogenin X and Y alleles. Lengths of DNA target for padlock probe detection were 40 and 33 bases of amelogenin X and Y alleles, respectively. Analysis of female DNA samples produced one L-RCA product from the AmelX padlock probe, whereas 2 different types of products were amplified from male DNA samples, each generated by AmelX and AmelY padlock probes. Utilization of padlock probes via L-RCA technique would provide an alternative tool for human sex determination in highly degraded DNA analysis in the future. © 2011 Elsevier Ireland Ltd. |
author2 |
Mahidol University |
author_facet |
Mahidol University Piyarat Thaijaroen Korapin Srisiri Rapee Boonplueang Nathinee Panvisavas |
format |
Article |
author |
Piyarat Thaijaroen Korapin Srisiri Rapee Boonplueang Nathinee Panvisavas |
author_sort |
Piyarat Thaijaroen |
title |
Human sex determination by amelogenin padlock probes |
title_short |
Human sex determination by amelogenin padlock probes |
title_full |
Human sex determination by amelogenin padlock probes |
title_fullStr |
Human sex determination by amelogenin padlock probes |
title_full_unstemmed |
Human sex determination by amelogenin padlock probes |
title_sort |
human sex determination by amelogenin padlock probes |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/11411 |
_version_ |
1763492284306817024 |