Novel insights into the biotin carboxylase domain reactions of pyruvate carboxylase from rhizobium etli

• Main Authors: Tonya N. Zeczycki, Ann L. Menefee, Abdussalam Adina-Zada, Sarawut Jitrapakdee, Kathy H. Surinya, John C. Wallace, Paul V. Attwood, Martin St. Maurice, W. Wallace Cleland
• Other Authors: University of Wisconsin Madison, Institute for Enzyme Research
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/11432
• Institution: Mahidol University

The catalytic mechanism of the MgATP-dependent carboxylation of biotin in the biotin carboxylase domain of pyruvate carboxylase from R. etli (RePC) is common to the biotin-dependent carboxylases. The current site-directed mutagenesis study has clarified the catalytic functions of several residues pr...