Insight into the carboxyl transferase domain mechanism of pyruvate carboxylase from Rhizobium etli

• Main Authors: Tonya N. Zeczycki, Martin St. Maurice, Sarawut Jitrapakdee, John C. Wallace, Paul V. Attwood, W. Wallace Cleland
• Other Authors: University of Wisconsin Madison, Institute for Enzyme Research
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/27222
• Institution: Mahidol University

The effects of mutations in the active site of the carboxyl transferase domain of Rhizobium etli pyruvate carboxylase have been determined for the forward reaction to form oxaloacetate, the reverse reaction to form MgATP, the oxamate-induced decarboxylation of oxaloacetate, the phosphorylation of Mg...