Probing the catalytic roles of arg548 and gln552 in the carboxyl transferase domain of the rhizobium etli pyruvate carboxylase by site-directed mutagenesis

• Main Authors: Saowapa Duangpan, Sarawut Jitrapakdee, Abdussalam Adina-Zada, Lindsay Byrne, Tonya N. Zeczycki, Martin St. Maurice, W. Wallace Cleland, John C. Wallace, Paul V. Attwood
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/28734

The roles of Arg548 and Gln552 residues in the active site of the carboxyl transferase domain of Rhizobium etli pyruvate carboxylase were investigated using site-directed mutagenesis. Mutation of Arg548 to alanine or glutamine resulted in the destabilization of the quaternary structure of the enzyme...