The role of the oligosaccharide binding cleft of rice BGlu1 in hydrolysis of cellooligosaccharides and in their synthesis by rice BGlu1 glycosynthase

Rice BGlu1 b-glucosidase nucleophile mutant E386G is a glycosynthase that can synthesize p-nitrophenyl (pNP)-cellooligosaccharides of up to 11 residues. The X-ray crystal structures of the E386G glycosynthase with and without a-glucosyl fluoride were solved and the a-glucosyl fluoride complex was fo...

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Bibliographic Details
Main Authors: Salila Pengthaisong, Stephen G. Withers, Buabarn Kuaprasert, Jisnuson Svasti, James R. Ketudat Cairns
Other Authors: Suranaree University of Technology
Format: Article
Published: 2018
Subjects:
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/13785
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Institution: Mahidol University