β-Glucosidase, exo-β-glucanase and pyridoxine transglucosylase activities of rice BGlu1

• Main Authors: Rodjana Opassiri, Yanling Hua, Onnop Wara-Aswapati, Takashi Akiyama, Jisnuson Svasti, Asim Esen, James R. Ketudat Cairns
• Other Authors: Suranaree University of Technology
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/21204
• Institution: Mahidol University

The bglu1 cDNA for a β-glucosidase cloned from rice (Oryza sativa L.) seedlings was expressed as a soluble and active protein in Escherichia coli and designated BGlu1. This enzyme hydrolysed β-1,4-linked oligosaccharides with increasing catalytic efficiency (kcat/Km) values as the DP (degree of poly...