β-Glucosidase, exo-β-glucanase and pyridoxine transglucosylase activities of rice BGlu1

The bglu1 cDNA for a β-glucosidase cloned from rice (Oryza sativa L.) seedlings was expressed as a soluble and active protein in Escherichia coli and designated BGlu1. This enzyme hydrolysed β-1,4-linked oligosaccharides with increasing catalytic efficiency (kcat/Km) values as the DP (degree of poly...

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Main Authors: Rodjana Opassiri, Yanling Hua, Onnop Wara-Aswapati, Takashi Akiyama, Jisnuson Svasti, Asim Esen, James R. Ketudat Cairns
Other Authors: Suranaree University of Technology
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/21204
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spelling th-mahidol.212042018-07-24T10:37:56Z β-Glucosidase, exo-β-glucanase and pyridoxine transglucosylase activities of rice BGlu1 Rodjana Opassiri Yanling Hua Onnop Wara-Aswapati Takashi Akiyama Jisnuson Svasti Asim Esen James R. Ketudat Cairns Suranaree University of Technology Hokkaido Agricultural Research Center, NARO Mahidol University Virginia Polytechnic Institute and State University Biochemistry, Genetics and Molecular Biology The bglu1 cDNA for a β-glucosidase cloned from rice (Oryza sativa L.) seedlings was expressed as a soluble and active protein in Escherichia coli and designated BGlu1. This enzyme hydrolysed β-1,4-linked oligosaccharides with increasing catalytic efficiency (kcat/Km) values as the DP (degree of polymerization) increased from 2 to 6. In contrast, hydrolysis of β-1,3-linked oligosaccharides decreased from DP 2 to 3, and polymers with a DP greater than 3 were not hydrolysed. The enzyme also hydrolysed p-nitrophenyl β-D-glycosides and some natural glucosides but with lower catalytic efficiency than β-linked oligosaccharides. Pyridoxine 5′-O-β-D- glucoside was the most efficiently hydrolysed natural glycoside tested. BGlu1 also had high transglucosylation activity towards pyridoxine, producing pyridoxine 5′-O-β-D-glucopyranoside in the presence of the glucose donor p-nitrophenyl β-D-glucoside. 2018-07-24T03:37:56Z 2018-07-24T03:37:56Z 2004-04-01 Article Biochemical Journal. Vol.379, No.1 (2004), 125-131 10.1042/BJ20031485 02646021 2-s2.0-1842866212 https://repository.li.mahidol.ac.th/handle/123456789/21204 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=1842866212&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
spellingShingle Biochemistry, Genetics and Molecular Biology
Rodjana Opassiri
Yanling Hua
Onnop Wara-Aswapati
Takashi Akiyama
Jisnuson Svasti
Asim Esen
James R. Ketudat Cairns
β-Glucosidase, exo-β-glucanase and pyridoxine transglucosylase activities of rice BGlu1
description The bglu1 cDNA for a β-glucosidase cloned from rice (Oryza sativa L.) seedlings was expressed as a soluble and active protein in Escherichia coli and designated BGlu1. This enzyme hydrolysed β-1,4-linked oligosaccharides with increasing catalytic efficiency (kcat/Km) values as the DP (degree of polymerization) increased from 2 to 6. In contrast, hydrolysis of β-1,3-linked oligosaccharides decreased from DP 2 to 3, and polymers with a DP greater than 3 were not hydrolysed. The enzyme also hydrolysed p-nitrophenyl β-D-glycosides and some natural glucosides but with lower catalytic efficiency than β-linked oligosaccharides. Pyridoxine 5′-O-β-D- glucoside was the most efficiently hydrolysed natural glycoside tested. BGlu1 also had high transglucosylation activity towards pyridoxine, producing pyridoxine 5′-O-β-D-glucopyranoside in the presence of the glucose donor p-nitrophenyl β-D-glucoside.
author2 Suranaree University of Technology
author_facet Suranaree University of Technology
Rodjana Opassiri
Yanling Hua
Onnop Wara-Aswapati
Takashi Akiyama
Jisnuson Svasti
Asim Esen
James R. Ketudat Cairns
format Article
author Rodjana Opassiri
Yanling Hua
Onnop Wara-Aswapati
Takashi Akiyama
Jisnuson Svasti
Asim Esen
James R. Ketudat Cairns
author_sort Rodjana Opassiri
title β-Glucosidase, exo-β-glucanase and pyridoxine transglucosylase activities of rice BGlu1
title_short β-Glucosidase, exo-β-glucanase and pyridoxine transglucosylase activities of rice BGlu1
title_full β-Glucosidase, exo-β-glucanase and pyridoxine transglucosylase activities of rice BGlu1
title_fullStr β-Glucosidase, exo-β-glucanase and pyridoxine transglucosylase activities of rice BGlu1
title_full_unstemmed β-Glucosidase, exo-β-glucanase and pyridoxine transglucosylase activities of rice BGlu1
title_sort β-glucosidase, exo-β-glucanase and pyridoxine transglucosylase activities of rice bglu1
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/21204
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