Ni 2 +-activated glyoxalase i from Escherichia coli: Substrate specificity, kinetic isotope effects and evolution within the βαβββ superfamily

• Main Authors: Kadia Y. Mullings, Nicole Sukdeo, Uthaiwan Suttisansanee, Yanhong Ran, John F. Honek
• Other Authors: University of Waterloo
• Format: Conference or Workshop Item
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemistry
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/13790
• Institution: Mahidol University

The Escherichia coli glyoxalase system consists of the metalloenzymes glyoxalase I and glyoxalase II. Little is known regarding Ni 2 + -activated E. coli glyoxalase I substrate specificity, its thiol cofactor preference, the presence or absence of any substrate kinetic isotope effects on the enzyme...