Cloned cDNA of A/swine/Iowa/15/1930 internal genes as a candidate backbone for reverse genetics vaccine against influenza A viruses

• Main Authors: Porntippa Lekcharoensuk, Witthawat Wiriyarat, Nantawan Petcharat, Chalermpol Lekcharoensuk, Prasert Auewarakul, Juergen A. Richt
• Other Authors: Kasetsart University
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology; Medicine; Veterinary
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/13801
• Institution: Mahidol University

Reverse genetics viruses for influenza vaccine production usually utilize the internal genes of the egg-adapted A/Puerto Rico/8/34 (PR8) strain. This egg-adapted strain provides high production yield in embryonated eggs but does not necessarily give the best yield in mammalian cell culture. In order...