The serotype-independent but concentrationdependent enhancing antibodies among Thai dengue patients

• Main Authors: Chai Teerasut, Huan Yao Lei, Khuendee Natthakorn, Akanitt Jittmittraphap, Suwich Thammapalo, Prasong Witayathawornwong, Ya Tin Lin, Richard G. Jarman, Pathom Sawanpanyalert, Wipawee Jampangern, Kriengsak Limkittikul
• Other Authors: Thailand Ministry of Public Health
• Format: Article
• Published: 2018
• Subjects: Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/14546
• Institution: Mahidol University

Antibody-dependent enhancement of infection (ADE) is central to explaining the development of severe disease at the end of post-dengue virus infection. Non-neutralizing anti-dengue antibodies bound to the dengue virion enhances the virus entrance into the target cells via the Fc receptor. The titer of enhancing antibodies in dengue patients is not determined during dengue virus infection. Sensitive flow cytometry detecting dengue virus-infected K562 cells was used to quantitate enhancing activity among Thai DF and DHF patients against four serotypes and the patient's dengue isolate. The titer was defined as the reciprocal of the final dilution that loses enhancing activity. The serum of Thai patients confirmed to have dengue infection were found to have high titers of enhancing antibodies and increased gradually through the convalescent phase of infection. The enhancing antibody titers were not different among the four serotypes or from the infecting isolate. The anti-dengue antibodies from dengue patients can enhance dengue virus infections in a concentration-dependent, serotype-independent manner.