Serine hydroxymethyltransferase from pyrimethamine-sensitive and -resistant strains of Plasmodium chabaudi
Serine hydroxymethyltransferase (EC 2.1.2.1) was partially purified from a pyrimethamine sensitive strain of Plasmodium chabaudi. K m values of 2.91 and 1.08 mM were determined for tetrahydrofolate and serine, respectively. The effects of pH, of temperature and of some potential inhibitors were dete...
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th-mahidol.157112018-06-14T16:14:04Z Serine hydroxymethyltransferase from pyrimethamine-sensitive and -resistant strains of Plasmodium chabaudi Pintip Ruenwongsa Metta Luanvararat William J. O'Sullivan Mahidol University Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Serine hydroxymethyltransferase (EC 2.1.2.1) was partially purified from a pyrimethamine sensitive strain of Plasmodium chabaudi. K m values of 2.91 and 1.08 mM were determined for tetrahydrofolate and serine, respectively. The effects of pH, of temperature and of some potential inhibitors were determined. The enzyme was also partially purified from a pyrimethamine-resistant strain of P. chabaudi and subjected to the same regime. No differences between the enzymes from the two sources could be detected. It would appear that the changes in properties in the enzymes dihydrofolate reductase and thymidylate synthetase associated with the development of drug resistance in P. chabaudi were not reflected in any obvious alterations in serine hydroxymethyltransferase. © 1989. 2018-06-14T09:12:52Z 2018-06-14T09:12:52Z 1989-03-15 Article Molecular and Biochemical Parasitology. Vol.33, No.3 (1989), 265-271 10.1016/0166-6851(89)90088-1 01666851 2-s2.0-0024596559 https://repository.li.mahidol.ac.th/handle/123456789/15711 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0024596559&origin=inward |
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Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Pintip Ruenwongsa Metta Luanvararat William J. O'Sullivan Serine hydroxymethyltransferase from pyrimethamine-sensitive and -resistant strains of Plasmodium chabaudi |
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Serine hydroxymethyltransferase (EC 2.1.2.1) was partially purified from a pyrimethamine sensitive strain of Plasmodium chabaudi. K m values of 2.91 and 1.08 mM were determined for tetrahydrofolate and serine, respectively. The effects of pH, of temperature and of some potential inhibitors were determined. The enzyme was also partially purified from a pyrimethamine-resistant strain of P. chabaudi and subjected to the same regime. No differences between the enzymes from the two sources could be detected. It would appear that the changes in properties in the enzymes dihydrofolate reductase and thymidylate synthetase associated with the development of drug resistance in P. chabaudi were not reflected in any obvious alterations in serine hydroxymethyltransferase. © 1989. |
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Mahidol University |
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Mahidol University Pintip Ruenwongsa Metta Luanvararat William J. O'Sullivan |
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Article |
author |
Pintip Ruenwongsa Metta Luanvararat William J. O'Sullivan |
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Pintip Ruenwongsa |
title |
Serine hydroxymethyltransferase from pyrimethamine-sensitive and -resistant strains of Plasmodium chabaudi |
title_short |
Serine hydroxymethyltransferase from pyrimethamine-sensitive and -resistant strains of Plasmodium chabaudi |
title_full |
Serine hydroxymethyltransferase from pyrimethamine-sensitive and -resistant strains of Plasmodium chabaudi |
title_fullStr |
Serine hydroxymethyltransferase from pyrimethamine-sensitive and -resistant strains of Plasmodium chabaudi |
title_full_unstemmed |
Serine hydroxymethyltransferase from pyrimethamine-sensitive and -resistant strains of Plasmodium chabaudi |
title_sort |
serine hydroxymethyltransferase from pyrimethamine-sensitive and -resistant strains of plasmodium chabaudi |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/15711 |
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1763494938551517184 |