Transgene copy number-dependent rescue of murine β-globin knockout mice carrying a 183 kb human β-globin BAC genomic fragment

• Main Authors: Jim Vadolas, Hady Wardan, Marco Bosmans, Faten Zaibak, Duangporn Jamsai, Lucille Voullaire, Robert Williamson, Panos A. Ioannou
• Other Authors: University of Melbourne
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/16351
• Institution: Mahidol University

We report the generation and characterisation of the first transgenic mice exclusively expressing normal human β-globin (huβ-globin) from a 183 kb genomic fragment. Four independent lines were generated, each containing 2-6 copies of thehuβ-globin locus at a single integration site. Steady state lev...