An efficient virus concentration method and RT-nested PCR for detection of rotaviruses in environmental water samples
Water samples were concentrated by the modified adsorption-elution technique followed by speedVac reconcentration of the filter eluates. Reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) was used to detect rotavirus RNA in concentrates of the water. The detection limit of the ro...
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Main Authors: | , , , , , , |
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Other Authors: | |
Format: | Article |
Published: |
2018
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Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/16603 |
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Institution: | Mahidol University |