An efficient virus concentration method and RT-nested PCR for detection of rotaviruses in environmental water samples

• Main Authors: Leera Kittigul, Som Ekchaloemkiet, Fuangfa Utrarachkij, Kanokrat Siripanichgon, Dusit Sujirarat, Supornvit Pungchitton, Augsana Boonthum
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Immunology and Microbiology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/16603

Water samples were concentrated by the modified adsorption-elution technique followed by speedVac reconcentration of the filter eluates. Reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) was used to detect rotavirus RNA in concentrates of the water. The detection limit of the ro...