Construction of a bifunctional enzyme, lactate dehydrogenase/ galactose dehydrogenase, able to recycle NADH
The in frame fusion between the galdh gene and the Idh gene encoding a linker region of five amino acids has been expressed in E.coli. The purified protein displays both enzyme activities and has a subunit molecular weight of 68500. The hybrid protein folds into a hexameric complex. When co-expresse...
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Main Authors: | , , |
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Format: | Article |
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2018
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Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/17852 |
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Institution: | Mahidol University |
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