Interobserver reproducibility in determining p16 overexpression in cervical lesions: Use of a combined scoring method
Objectives: To evaluate interobserver reproducibility of a combined scoring method for immunohistochemical interpretation of p16 overexpression in cervical lesions. Materials and methods: p16 immunostaining was performed in cervical samples from 183 patients, including 69 normal, 42 low grade squamo...
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th-mahidol.190152018-07-12T09:49:01Z Interobserver reproducibility in determining p16 overexpression in cervical lesions: Use of a combined scoring method Songkhun Vinyuvat Anant Karalak Cheepsumon Suthipintawong Kobkul Tungsinmunkong Pilaiwan Kleebkaow Prasert Trivijitsilp Sumalee Siriaunkgul Surang Triratanachat Surapan Khunamornpong Tuenjai Chuangsuwanich Jongkolnee Settakorn Thailand Ministry of Public Health Rajavithi Hospital Prince of Songkla University Khon Kaen University Chulalongkorn University Chiang Mai University Mahidol University Biochemistry, Genetics and Molecular Biology Medicine Objectives: To evaluate interobserver reproducibility of a combined scoring method for immunohistochemical interpretation of p16 overexpression in cervical lesions. Materials and methods: p16 immunostaining was performed in cervical samples from 183 patients, including 69 normal, 42 low grade squamous intraepithelial lesions(LSIL), 36 high grade SIL (HSIL), and 36 squamous cell carcinomas(SCCAs). Each case was evaluated by a combined scoring method based on the percentage of positive cells (score 0-3), the intensitiy of staining (score 0-3), and the distribution pattern (score 0-2). Immunoexpression for p16 was considered as positive when the combined score was 4-8 and negative with a score of 0-3. Ten pathologists with varied experience in interpretating p16 immunostains evaluated each slide independently. Results: All normal cervical squamous epithelia (69/69) were uniformly negative for p16. All HSILs (36/36), all SCCAs (100/100), and all but one of the LSILs (40/41, 97.62%) showed positive expression. In 172 of 183 cases (93.99%), p16 interpretation was concordant with all pathologists. Eleven cases with discordant results included 10 LSILs and 1 normal mucosa sample. Percentage of agreement of each pathologist pair ranged from 96.7-100% (mean 98.1 ± 0.96%) with mean kappa value of 0.96 ± 0.0201 (range 0.93-1.000). Conclusion: The proposed combined scoring method shows good reproducibility among the participating pathologists and good correlation with the histologic diagnosis. This method may be a useful guide in the interpretation of p16 expression in cervical epithelial lesions. 2018-07-12T02:20:53Z 2018-07-12T02:20:53Z 2008-01-01 Article Asian Pacific Journal of Cancer Prevention. Vol.9, No.4 (2008), 653-657 2476762X 15137368 2-s2.0-66149089609 https://repository.li.mahidol.ac.th/handle/123456789/19015 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=66149089609&origin=inward |
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Biochemistry, Genetics and Molecular Biology Medicine Songkhun Vinyuvat Anant Karalak Cheepsumon Suthipintawong Kobkul Tungsinmunkong Pilaiwan Kleebkaow Prasert Trivijitsilp Sumalee Siriaunkgul Surang Triratanachat Surapan Khunamornpong Tuenjai Chuangsuwanich Jongkolnee Settakorn Interobserver reproducibility in determining p16 overexpression in cervical lesions: Use of a combined scoring method |
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Objectives: To evaluate interobserver reproducibility of a combined scoring method for immunohistochemical interpretation of p16 overexpression in cervical lesions. Materials and methods: p16 immunostaining was performed in cervical samples from 183 patients, including 69 normal, 42 low grade squamous intraepithelial lesions(LSIL), 36 high grade SIL (HSIL), and 36 squamous cell carcinomas(SCCAs). Each case was evaluated by a combined scoring method based on the percentage of positive cells (score 0-3), the intensitiy of staining (score 0-3), and the distribution pattern (score 0-2). Immunoexpression for p16 was considered as positive when the combined score was 4-8 and negative with a score of 0-3. Ten pathologists with varied experience in interpretating p16 immunostains evaluated each slide independently. Results: All normal cervical squamous epithelia (69/69) were uniformly negative for p16. All HSILs (36/36), all SCCAs (100/100), and all but one of the LSILs (40/41, 97.62%) showed positive expression. In 172 of 183 cases (93.99%), p16 interpretation was concordant with all pathologists. Eleven cases with discordant results included 10 LSILs and 1 normal mucosa sample. Percentage of agreement of each pathologist pair ranged from 96.7-100% (mean 98.1 ± 0.96%) with mean kappa value of 0.96 ± 0.0201 (range 0.93-1.000). Conclusion: The proposed combined scoring method shows good reproducibility among the participating pathologists and good correlation with the histologic diagnosis. This method may be a useful guide in the interpretation of p16 expression in cervical epithelial lesions. |
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Thailand Ministry of Public Health |
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Thailand Ministry of Public Health Songkhun Vinyuvat Anant Karalak Cheepsumon Suthipintawong Kobkul Tungsinmunkong Pilaiwan Kleebkaow Prasert Trivijitsilp Sumalee Siriaunkgul Surang Triratanachat Surapan Khunamornpong Tuenjai Chuangsuwanich Jongkolnee Settakorn |
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Article |
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Songkhun Vinyuvat Anant Karalak Cheepsumon Suthipintawong Kobkul Tungsinmunkong Pilaiwan Kleebkaow Prasert Trivijitsilp Sumalee Siriaunkgul Surang Triratanachat Surapan Khunamornpong Tuenjai Chuangsuwanich Jongkolnee Settakorn |
author_sort |
Songkhun Vinyuvat |
title |
Interobserver reproducibility in determining p16 overexpression in cervical lesions: Use of a combined scoring method |
title_short |
Interobserver reproducibility in determining p16 overexpression in cervical lesions: Use of a combined scoring method |
title_full |
Interobserver reproducibility in determining p16 overexpression in cervical lesions: Use of a combined scoring method |
title_fullStr |
Interobserver reproducibility in determining p16 overexpression in cervical lesions: Use of a combined scoring method |
title_full_unstemmed |
Interobserver reproducibility in determining p16 overexpression in cervical lesions: Use of a combined scoring method |
title_sort |
interobserver reproducibility in determining p16 overexpression in cervical lesions: use of a combined scoring method |
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2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/19015 |
_version_ |
1763497222718095360 |