Detection and haplotype differentiation of Southeast Asian α-thalassemia using polymerase chain reaction and a piezoelectric biosensor immobilized with a single oligonucleotide probe

DNA-based diagnosis of α-thalassemias routinely relies on polymerase chain reaction (PCR) and gel electrophoresis. Here, we developed a new procedure for the detection and haplotype differentiation of Southeast Asian (SEA) α-thalassemia using a 3-primer system for PCR coupling with a DNA-based piezo...

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Main Authors: Phantip Vattanaviboon, Kulphassorn Sangseekhiow, Pranee Winichagoon, Chamras Promptmas
Other Authors: Mahidol University
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/19694
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spelling th-mahidol.196942018-07-12T09:43:40Z Detection and haplotype differentiation of Southeast Asian α-thalassemia using polymerase chain reaction and a piezoelectric biosensor immobilized with a single oligonucleotide probe Phantip Vattanaviboon Kulphassorn Sangseekhiow Pranee Winichagoon Chamras Promptmas Mahidol University Rajavithi Hospital The Institute of Science and Technology for Research and Development, Mahidol University Medicine DNA-based diagnosis of α-thalassemias routinely relies on polymerase chain reaction (PCR) and gel electrophoresis. Here, we developed a new procedure for the detection and haplotype differentiation of Southeast Asian (SEA) α-thalassemia using a 3-primer system for PCR coupling with a DNA-based piezoelectric biosensor. PCR products amplified from genomic DNA were differentiated directly by using a quartz crystal microbalance immobilized with a single oligonucleotide probe. The frequency changes after hybridization of the PCR products amplified from a representative sample of normal α-globin, SEA α-thalassemia heterozygote, and homozygote were 206 ± 11, 256 ± 5, and 307 ± 3 Hz, respectively. The fabricated biosensor was evaluated through an examination of 18 blind specimens. It could accurately discriminate between normal and SEA α-thalassemic samples, which suggests that this biosensor system is a promising alternative technique to detect SEA α-thalassemia because of its specificity and less hazardous exposure as compared with conventional methods. © 2008 Mosby, Inc. All rights reserved. 2018-07-12T02:43:40Z 2018-07-12T02:43:40Z 2008-05-01 Article Translational Research. Vol.151, No.5 (2008), 246-254 10.1016/j.trsl.2007.12.009 19315244 2-s2.0-42149149896 https://repository.li.mahidol.ac.th/handle/123456789/19694 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=42149149896&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Medicine
spellingShingle Medicine
Phantip Vattanaviboon
Kulphassorn Sangseekhiow
Pranee Winichagoon
Chamras Promptmas
Detection and haplotype differentiation of Southeast Asian α-thalassemia using polymerase chain reaction and a piezoelectric biosensor immobilized with a single oligonucleotide probe
description DNA-based diagnosis of α-thalassemias routinely relies on polymerase chain reaction (PCR) and gel electrophoresis. Here, we developed a new procedure for the detection and haplotype differentiation of Southeast Asian (SEA) α-thalassemia using a 3-primer system for PCR coupling with a DNA-based piezoelectric biosensor. PCR products amplified from genomic DNA were differentiated directly by using a quartz crystal microbalance immobilized with a single oligonucleotide probe. The frequency changes after hybridization of the PCR products amplified from a representative sample of normal α-globin, SEA α-thalassemia heterozygote, and homozygote were 206 ± 11, 256 ± 5, and 307 ± 3 Hz, respectively. The fabricated biosensor was evaluated through an examination of 18 blind specimens. It could accurately discriminate between normal and SEA α-thalassemic samples, which suggests that this biosensor system is a promising alternative technique to detect SEA α-thalassemia because of its specificity and less hazardous exposure as compared with conventional methods. © 2008 Mosby, Inc. All rights reserved.
author2 Mahidol University
author_facet Mahidol University
Phantip Vattanaviboon
Kulphassorn Sangseekhiow
Pranee Winichagoon
Chamras Promptmas
format Article
author Phantip Vattanaviboon
Kulphassorn Sangseekhiow
Pranee Winichagoon
Chamras Promptmas
author_sort Phantip Vattanaviboon
title Detection and haplotype differentiation of Southeast Asian α-thalassemia using polymerase chain reaction and a piezoelectric biosensor immobilized with a single oligonucleotide probe
title_short Detection and haplotype differentiation of Southeast Asian α-thalassemia using polymerase chain reaction and a piezoelectric biosensor immobilized with a single oligonucleotide probe
title_full Detection and haplotype differentiation of Southeast Asian α-thalassemia using polymerase chain reaction and a piezoelectric biosensor immobilized with a single oligonucleotide probe
title_fullStr Detection and haplotype differentiation of Southeast Asian α-thalassemia using polymerase chain reaction and a piezoelectric biosensor immobilized with a single oligonucleotide probe
title_full_unstemmed Detection and haplotype differentiation of Southeast Asian α-thalassemia using polymerase chain reaction and a piezoelectric biosensor immobilized with a single oligonucleotide probe
title_sort detection and haplotype differentiation of southeast asian α-thalassemia using polymerase chain reaction and a piezoelectric biosensor immobilized with a single oligonucleotide probe
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/19694
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