Detection and haplotype differentiation of Southeast Asian α-thalassemia using polymerase chain reaction and a piezoelectric biosensor immobilized with a single oligonucleotide probe

• Main Authors: Phantip Vattanaviboon, Kulphassorn Sangseekhiow, Pranee Winichagoon, Chamras Promptmas
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/19694

DNA-based diagnosis of α-thalassemias routinely relies on polymerase chain reaction (PCR) and gel electrophoresis. Here, we developed a new procedure for the detection and haplotype differentiation of Southeast Asian (SEA) α-thalassemia using a 3-primer system for PCR coupling with a DNA-based piezo...