In-vitro response of Plasmodium falciparum to the main alkaloids of Cinchona in northwestern Thailand
The blood schizontocidal activity of the four main Cinchona alkaloids against Plasmodium falciparum was compared in 46 fresh parasite isolates, using an in-vitro test measuring the drug-specific inhibition of schizont maturation. The studies were conducted in June-August 2001 at Mae Sot, northwester...
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th-mahidol.209982018-07-24T10:28:15Z In-vitro response of Plasmodium falciparum to the main alkaloids of Cinchona in northwestern Thailand Ariane Knauer Jeeraphat Sirichaisinthop Franz F. Reinthaler Gerhard Wiedermann Gunther Wernsdorfer Walther H. Wernsdorfer Karl-Franzens-Universitat Graz Mahidol University Off. of Vector-Borne Disease Control Universitat Wien Dept. Specific Prophylaxis/Trop. M. Medicine The blood schizontocidal activity of the four main Cinchona alkaloids against Plasmodium falciparum was compared in 46 fresh parasite isolates, using an in-vitro test measuring the drug-specific inhibition of schizont maturation. The studies were conducted in June-August 2001 at Mae Sot, northwestern Thailand, an area where quinine alone is no longer able to eliminate infections with P. falciparum. Quinidine showed the highest blood schizontocidal activity, followed by cinchonine, cinchonidine and finally quinine, which was identified as the least active compound. The isolates showed marked heterogeneity in their response to the Cinchona alkaloids. There was also high correlation of activity among all four alkaloids. The mean EC50values for quinine, quinidine, cinchonine and cinchonidine were 144 nM, 80 nM, 104 nM and 225 nM, respectively, and the EC99values 8040 nM, 861 nM, 1176 nM and 6531 nM. The EC99values for quinine and cinchonidine are beyond the therapeutic concentration range and those for quinidine within it. For cinchonine, values are likely to be within this range, but toxicological and pharmacokinetic studies on this compound are required for clarifying its potential future role in the treatment of falciparum malaria. 2018-07-24T03:28:15Z 2018-07-24T03:28:15Z 2003-12-01 Conference Paper Wiener Klinische Wochenschrift, Supplement. Vol.115, No.3 (2003), 39-44 03005178 2-s2.0-0842269874 https://repository.li.mahidol.ac.th/handle/123456789/20998 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0842269874&origin=inward |
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Medicine Ariane Knauer Jeeraphat Sirichaisinthop Franz F. Reinthaler Gerhard Wiedermann Gunther Wernsdorfer Walther H. Wernsdorfer In-vitro response of Plasmodium falciparum to the main alkaloids of Cinchona in northwestern Thailand |
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The blood schizontocidal activity of the four main Cinchona alkaloids against Plasmodium falciparum was compared in 46 fresh parasite isolates, using an in-vitro test measuring the drug-specific inhibition of schizont maturation. The studies were conducted in June-August 2001 at Mae Sot, northwestern Thailand, an area where quinine alone is no longer able to eliminate infections with P. falciparum. Quinidine showed the highest blood schizontocidal activity, followed by cinchonine, cinchonidine and finally quinine, which was identified as the least active compound. The isolates showed marked heterogeneity in their response to the Cinchona alkaloids. There was also high correlation of activity among all four alkaloids. The mean EC50values for quinine, quinidine, cinchonine and cinchonidine were 144 nM, 80 nM, 104 nM and 225 nM, respectively, and the EC99values 8040 nM, 861 nM, 1176 nM and 6531 nM. The EC99values for quinine and cinchonidine are beyond the therapeutic concentration range and those for quinidine within it. For cinchonine, values are likely to be within this range, but toxicological and pharmacokinetic studies on this compound are required for clarifying its potential future role in the treatment of falciparum malaria. |
author2 |
Karl-Franzens-Universitat Graz |
author_facet |
Karl-Franzens-Universitat Graz Ariane Knauer Jeeraphat Sirichaisinthop Franz F. Reinthaler Gerhard Wiedermann Gunther Wernsdorfer Walther H. Wernsdorfer |
format |
Conference or Workshop Item |
author |
Ariane Knauer Jeeraphat Sirichaisinthop Franz F. Reinthaler Gerhard Wiedermann Gunther Wernsdorfer Walther H. Wernsdorfer |
author_sort |
Ariane Knauer |
title |
In-vitro response of Plasmodium falciparum to the main alkaloids of Cinchona in northwestern Thailand |
title_short |
In-vitro response of Plasmodium falciparum to the main alkaloids of Cinchona in northwestern Thailand |
title_full |
In-vitro response of Plasmodium falciparum to the main alkaloids of Cinchona in northwestern Thailand |
title_fullStr |
In-vitro response of Plasmodium falciparum to the main alkaloids of Cinchona in northwestern Thailand |
title_full_unstemmed |
In-vitro response of Plasmodium falciparum to the main alkaloids of Cinchona in northwestern Thailand |
title_sort |
in-vitro response of plasmodium falciparum to the main alkaloids of cinchona in northwestern thailand |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/20998 |
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1763489437935730688 |