Cucurbitacin B causes increased radiation sensitivity of human breast cancer cells via G2/M cell cycle arrest
Purpose. To explore the effects of cucurbitacin B on the radiation survival of human breast cancer cells and to elucidate the cellular mechanism of radiosensitization if any. Materials and Methods. Human breast carcinoma cell lines were treated with cucurbitacin B before irradiation with 0–10Gy of...
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Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Mahidol University. Faculty of Medical Technology
2013
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Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/2100 |
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Institution: | Mahidol University |
Language: | English |
Summary: | Purpose. To explore the effects of cucurbitacin B on the radiation survival of human breast cancer cells and to elucidate the cellular
mechanism of radiosensitization if any. Materials and Methods. Human breast carcinoma cell lines were treated with cucurbitacin
B before irradiation with 0–10Gy of 137Cs gamma rays. The effect of cucurbitacin B on cell-survival following irradiation was
evaluated by colony-forming assay. Cell cycle distributions were investigated using flow cytometry. Real-time PCR and western
blots were performed to investigate the expression of cell cycle checkpoints. Results. Cucurbitacin B inhibited breast cancer cell
proliferation in a dose-dependent manner. Only MDA-MB-231 and MCF7:5C cells but not SKBR-3 cells were radiosensitized by
cucurbitacin B. Flow cytometric analysis for DNA content indicated that cucurbitacin B resulted in G2/M arrest in MDA-MB-231
andMCF7:5C but not SKBR-3 cells.Moreover, Real-time PCR and western blot analysis demonstrated upregulated p21 expression
before irradiation, a likely cause of the cell cycle arrest. Conclusion. Taken together, these findings suggest that cucurbitacin B causes
radiosensitization of some breast cancer cells, and that cucurbitacin B induced G2/M arrest is an importantmechanism. Therefore,
combinations of cucurbitacin B with radiotherapy may be appropriate for experimental breast cancer treatment. |
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