Cucurbitacin B causes increased radiation sensitivity of human breast cancer cells via G2/M cell cycle arrest
Purpose. To explore the effects of cucurbitacin B on the radiation survival of human breast cancer cells and to elucidate the cellular mechanism of radiosensitization if any. Materials and Methods. Human breast carcinoma cell lines were treated with cucurbitacin B before irradiation with 0–10Gy of...
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th-mahidol.21002023-04-12T15:27:30Z Cucurbitacin B causes increased radiation sensitivity of human breast cancer cells via G2/M cell cycle arrest Suwit Duangmano Phorntip Sae-lim Apichart Suksamrarn Pimpichaya Patmasiriwat Frederick E. Domann Cucurbitacin B Human Breast Cancer Cell Cycle Arrest Radiation Sensitivity Purpose. To explore the effects of cucurbitacin B on the radiation survival of human breast cancer cells and to elucidate the cellular mechanism of radiosensitization if any. Materials and Methods. Human breast carcinoma cell lines were treated with cucurbitacin B before irradiation with 0–10Gy of 137Cs gamma rays. The effect of cucurbitacin B on cell-survival following irradiation was evaluated by colony-forming assay. Cell cycle distributions were investigated using flow cytometry. Real-time PCR and western blots were performed to investigate the expression of cell cycle checkpoints. Results. Cucurbitacin B inhibited breast cancer cell proliferation in a dose-dependent manner. Only MDA-MB-231 and MCF7:5C cells but not SKBR-3 cells were radiosensitized by cucurbitacin B. Flow cytometric analysis for DNA content indicated that cucurbitacin B resulted in G2/M arrest in MDA-MB-231 andMCF7:5C but not SKBR-3 cells.Moreover, Real-time PCR and western blot analysis demonstrated upregulated p21 expression before irradiation, a likely cause of the cell cycle arrest. Conclusion. Taken together, these findings suggest that cucurbitacin B causes radiosensitization of some breast cancer cells, and that cucurbitacin B induced G2/M arrest is an importantmechanism. Therefore, combinations of cucurbitacin B with radiotherapy may be appropriate for experimental breast cancer treatment. 2013-06-17T07:40:51Z 2017-06-20T16:24:50Z 2013-06-17T07:40:51Z 2017-06-20T16:24:50Z 2013-06-17 Article Journal of Oncology. 2012, ID 601682 https://repository.li.mahidol.ac.th/handle/123456789/2100 eng application/pdf Mahidol University. Faculty of Medical Technology |
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Cucurbitacin B Human Breast Cancer Cell Cycle Arrest Radiation Sensitivity |
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Cucurbitacin B Human Breast Cancer Cell Cycle Arrest Radiation Sensitivity Suwit Duangmano Phorntip Sae-lim Apichart Suksamrarn Pimpichaya Patmasiriwat Frederick E. Domann Cucurbitacin B causes increased radiation sensitivity of human breast cancer cells via G2/M cell cycle arrest |
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Purpose. To explore the effects of cucurbitacin B on the radiation survival of human breast cancer cells and to elucidate the cellular
mechanism of radiosensitization if any. Materials and Methods. Human breast carcinoma cell lines were treated with cucurbitacin
B before irradiation with 0–10Gy of 137Cs gamma rays. The effect of cucurbitacin B on cell-survival following irradiation was
evaluated by colony-forming assay. Cell cycle distributions were investigated using flow cytometry. Real-time PCR and western
blots were performed to investigate the expression of cell cycle checkpoints. Results. Cucurbitacin B inhibited breast cancer cell
proliferation in a dose-dependent manner. Only MDA-MB-231 and MCF7:5C cells but not SKBR-3 cells were radiosensitized by
cucurbitacin B. Flow cytometric analysis for DNA content indicated that cucurbitacin B resulted in G2/M arrest in MDA-MB-231
andMCF7:5C but not SKBR-3 cells.Moreover, Real-time PCR and western blot analysis demonstrated upregulated p21 expression
before irradiation, a likely cause of the cell cycle arrest. Conclusion. Taken together, these findings suggest that cucurbitacin B causes
radiosensitization of some breast cancer cells, and that cucurbitacin B induced G2/M arrest is an importantmechanism. Therefore,
combinations of cucurbitacin B with radiotherapy may be appropriate for experimental breast cancer treatment. |
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Article |
author |
Suwit Duangmano Phorntip Sae-lim Apichart Suksamrarn Pimpichaya Patmasiriwat Frederick E. Domann |
author_facet |
Suwit Duangmano Phorntip Sae-lim Apichart Suksamrarn Pimpichaya Patmasiriwat Frederick E. Domann |
author_sort |
Suwit Duangmano |
title |
Cucurbitacin B causes increased radiation sensitivity of human breast cancer cells via G2/M cell cycle arrest |
title_short |
Cucurbitacin B causes increased radiation sensitivity of human breast cancer cells via G2/M cell cycle arrest |
title_full |
Cucurbitacin B causes increased radiation sensitivity of human breast cancer cells via G2/M cell cycle arrest |
title_fullStr |
Cucurbitacin B causes increased radiation sensitivity of human breast cancer cells via G2/M cell cycle arrest |
title_full_unstemmed |
Cucurbitacin B causes increased radiation sensitivity of human breast cancer cells via G2/M cell cycle arrest |
title_sort |
cucurbitacin b causes increased radiation sensitivity of human breast cancer cells via g2/m cell cycle arrest |
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Mahidol University. Faculty of Medical Technology |
publishDate |
2013 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/2100 |
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1781415301662900224 |