Spectrophotometric enzymatic cycling method using L-glutamate dehydrogenase and D-phenylglycine aminotransferase for determination of L-glutamate in foods

This report describes a new spectrophotometric method capable of determining low levels of L-glutamate. The assay is based on substrate cycling between L-glutamate dehydrogenase (GlDH) and the novel enzyme D-phenylglycine aminotransferase (D-PhgAT). In this system, GlDH converts L-glutamate to 2-oxo...

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Bibliographic Details
Main Authors: Wanida Khampha, Vithaya Meevootisom, Suthep Wiyakrutta
Other Authors: Mahidol University
Format: Article
Published: 2018
Subjects:
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/21166
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Institution: Mahidol University