On-chip microfluidic systems for determination of L-glutamate based on enzymatic recycling of substrate

• Main Authors: Wanida Laiwattanapaisal, J. Yakovleva, M. Bengtsson, T. Laurell, S. Wiyakrutta, V. Meevootisom, O. Chailapakul, J. Emnéus
• Other Authors: Chulalongkorn University
• Format: Conference or Workshop Item
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemistry; Materials Science; Physics and Astronomy
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/27244
• Institution: Mahidol University

Two microfluidic systems have been developed for specific analysis of L-glutamate in food based on substrate recycling fluorescence detection. L-glutamate dehydrogenase and a novel enzyme, D-phenylglycine aminotransferase, were covalently immobilized on (i) the surface of silicon microchips containi...