Kinetic Characterization of Yeast Pyruvate Carboxylase Isozyme Pyc1 and the Pyc1 Mutant, C249A

• Main Authors: Joy P. Branson, Mark Nezic, Sarawut Jitrapakdee, John C. Wallace, Paul V. Attwood
• Other Authors: University of Western Australia
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/21227
• Institution: Mahidol University

The yeast Pyc1 isoform of pyruvate carboxylase has been further characterized and shown to differ from the Pyc2 isoform in its Ka for K+ activation. Pyc1 differs from chicken liver pyruvate carboxylase in the lack of effect of acetyl-CoA on ADP phosphorylation by carbamoyl phosphate, which may be a...