Transformation of Aspergillus oryzae with a dominant selectable marker

Aspergillus oryzae strain U1638 has been transformed to phleomycin resistance using a phleomycin based vector. Protoplasts were generated using Novozyme 234 and regenerated on sucrose-stabilized minimal medium containing 200 μg ml-1phleomycin. A frequency of 3 to 7 transformants per 106viable protop...

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Bibliographic Details
Main Authors: S. Cheevadhanarak, G. Saunders, D. V. Renno, T. W. Flegel, G. Holt
Other Authors: University of Westminster
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/22005
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Institution: Mahidol University