Transformation of Aspergillus oryzae with a dominant selectable marker

Transformation of Aspergillus oryzae with a dominant selectable marker

Aspergillus oryzae strain U1638 has been transformed to phleomycin resistance using a phleomycin based vector. Protoplasts were generated using Novozyme 234 and regenerated on sucrose-stabilized minimal medium containing 200 μg ml-1phleomycin. A frequency of 3 to 7 transformants per 106viable protop...

Full description

Saved in:
Bibliographic Details
Main Authors: S. Cheevadhanarak, G. Saunders, D. V. Renno, T. W. Flegel, G. Holt
Other Authors: University of Westminster
Format: Article
Published: 2018
Subjects:
Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/22005
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Mahidol University
id th-mahidol.22005
record_format dspace
spelling th-mahidol.220052018-08-10T15:32:05Z Transformation of Aspergillus oryzae with a dominant selectable marker S. Cheevadhanarak G. Saunders D. V. Renno T. W. Flegel G. Holt University of Westminster Mahidol University Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Aspergillus oryzae strain U1638 has been transformed to phleomycin resistance using a phleomycin based vector. Protoplasts were generated using Novozyme 234 and regenerated on sucrose-stabilized minimal medium containing 200 μg ml-1phleomycin. A frequency of 3 to 7 transformants per 106viable protoplasts was obtained. All transformants tested showed increased resistance to phleomycin when subcultured onto selective medium and the mitotic stability of the transformants was 100% after growth without selective pressure. The vector sequences appeared to be integrated into the A. oryzae U1638 genome at multiple sites. © 1991. 2018-08-10T08:30:49Z 2018-08-10T08:30:49Z 1991-01-01 Article Journal of Biotechnology. Vol.19, No.1 (1991), 117-122 10.1016/0168-1656(91)90079-B 01681656 2-s2.0-0025736709 https://repository.li.mahidol.ac.th/handle/123456789/22005 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0025736709&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
spellingShingle Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
S. Cheevadhanarak
G. Saunders
D. V. Renno
T. W. Flegel
G. Holt
Transformation of Aspergillus oryzae with a dominant selectable marker
description Aspergillus oryzae strain U1638 has been transformed to phleomycin resistance using a phleomycin based vector. Protoplasts were generated using Novozyme 234 and regenerated on sucrose-stabilized minimal medium containing 200 μg ml-1phleomycin. A frequency of 3 to 7 transformants per 106viable protoplasts was obtained. All transformants tested showed increased resistance to phleomycin when subcultured onto selective medium and the mitotic stability of the transformants was 100% after growth without selective pressure. The vector sequences appeared to be integrated into the A. oryzae U1638 genome at multiple sites. © 1991.
author2 University of Westminster
author_facet University of Westminster
S. Cheevadhanarak
G. Saunders
D. V. Renno
T. W. Flegel
G. Holt
format Article
author S. Cheevadhanarak
G. Saunders
D. V. Renno
T. W. Flegel
G. Holt
author_sort S. Cheevadhanarak
title Transformation of Aspergillus oryzae with a dominant selectable marker
title_short Transformation of Aspergillus oryzae with a dominant selectable marker
title_full Transformation of Aspergillus oryzae with a dominant selectable marker
title_fullStr Transformation of Aspergillus oryzae with a dominant selectable marker
title_full_unstemmed Transformation of Aspergillus oryzae with a dominant selectable marker
title_sort transformation of aspergillus oryzae with a dominant selectable marker
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/22005
_version_ 1763494768416915456

Similar Items