Enzyme-linked staining method for light microscopic detection of antibodies to parasite antigens on the membrane of Plasmodium falciparum infected erythrocytes
Indirect erythrocyte membrane immunofluorescence (EMIF), a standard method for detection of antibodies to the P. falciparum blood stage vaccine candidate antigen Pf155/RESA, has been adapted to light microscopy by enzyme-linked immunostaining of the erythrocyte membrane, using alkaline phosphatase a...
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th-mahidol.225242018-08-10T16:01:37Z Enzyme-linked staining method for light microscopic detection of antibodies to parasite antigens on the membrane of Plasmodium falciparum infected erythrocytes Rachanee Udomsangpetch Asli Kulane Klavs Berzins Hedvig Perlmann Peter Perlmann Birgitta Wåhlin Flyg Mahidol University Stockholms universitet Somali National University Agricultural and Biological Sciences Immunology and Microbiology Medicine Veterinary Indirect erythrocyte membrane immunofluorescence (EMIF), a standard method for detection of antibodies to the P. falciparum blood stage vaccine candidate antigen Pf155/RESA, has been adapted to light microscopy by enzyme-linked immunostaining of the erythrocyte membrane, using alkaline phosphatase and chromogenic substrate. This method gives a dark blue staining of the membranes of ring infected erythrocytes. Results obtained with 70 African sera in EMIF and in enzyme immunostaining correlated well although the enzyme based method sometimes resulted in higher antibody titers and appeared to be slightly more sensitive. Similar results were obtained when comparing immunofluorescence with enzyme immunostaining for detection of antibodies to intraerythrocytic parasite antigens. The enzyme linked immunostaining described is simple and fast and does not require expensive equipment and should, thus, be well suited for use in laboratories with limited resources or under field conditions. © 1993. 2018-08-10T08:53:59Z 2018-08-10T08:53:59Z 1993-01-01 Article Acta Tropica. Vol.55, No.1-2 (1993), 79-86 10.1016/0001-706X(93)90050-L 0001706X 2-s2.0-0027364882 https://repository.li.mahidol.ac.th/handle/123456789/22524 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0027364882&origin=inward |
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Agricultural and Biological Sciences Immunology and Microbiology Medicine Veterinary Rachanee Udomsangpetch Asli Kulane Klavs Berzins Hedvig Perlmann Peter Perlmann Birgitta Wåhlin Flyg Enzyme-linked staining method for light microscopic detection of antibodies to parasite antigens on the membrane of Plasmodium falciparum infected erythrocytes |
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Indirect erythrocyte membrane immunofluorescence (EMIF), a standard method for detection of antibodies to the P. falciparum blood stage vaccine candidate antigen Pf155/RESA, has been adapted to light microscopy by enzyme-linked immunostaining of the erythrocyte membrane, using alkaline phosphatase and chromogenic substrate. This method gives a dark blue staining of the membranes of ring infected erythrocytes. Results obtained with 70 African sera in EMIF and in enzyme immunostaining correlated well although the enzyme based method sometimes resulted in higher antibody titers and appeared to be slightly more sensitive. Similar results were obtained when comparing immunofluorescence with enzyme immunostaining for detection of antibodies to intraerythrocytic parasite antigens. The enzyme linked immunostaining described is simple and fast and does not require expensive equipment and should, thus, be well suited for use in laboratories with limited resources or under field conditions. © 1993. |
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Mahidol University |
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Mahidol University Rachanee Udomsangpetch Asli Kulane Klavs Berzins Hedvig Perlmann Peter Perlmann Birgitta Wåhlin Flyg |
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Article |
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Rachanee Udomsangpetch Asli Kulane Klavs Berzins Hedvig Perlmann Peter Perlmann Birgitta Wåhlin Flyg |
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Rachanee Udomsangpetch |
title |
Enzyme-linked staining method for light microscopic detection of antibodies to parasite antigens on the membrane of Plasmodium falciparum infected erythrocytes |
title_short |
Enzyme-linked staining method for light microscopic detection of antibodies to parasite antigens on the membrane of Plasmodium falciparum infected erythrocytes |
title_full |
Enzyme-linked staining method for light microscopic detection of antibodies to parasite antigens on the membrane of Plasmodium falciparum infected erythrocytes |
title_fullStr |
Enzyme-linked staining method for light microscopic detection of antibodies to parasite antigens on the membrane of Plasmodium falciparum infected erythrocytes |
title_full_unstemmed |
Enzyme-linked staining method for light microscopic detection of antibodies to parasite antigens on the membrane of Plasmodium falciparum infected erythrocytes |
title_sort |
enzyme-linked staining method for light microscopic detection of antibodies to parasite antigens on the membrane of plasmodium falciparum infected erythrocytes |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/22524 |
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1763493551236186112 |