Enzyme-linked staining method for light microscopic detection of antibodies to parasite antigens on the membrane of Plasmodium falciparum infected erythrocytes

• Main Authors: Rachanee Udomsangpetch, Asli Kulane, Klavs Berzins, Hedvig Perlmann, Peter Perlmann, Birgitta Wåhlin Flyg
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Agricultural and Biological Sciences; Immunology and Microbiology; Medicine; Veterinary
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/22524

Indirect erythrocyte membrane immunofluorescence (EMIF), a standard method for detection of antibodies to the P. falciparum blood stage vaccine candidate antigen Pf155/RESA, has been adapted to light microscopy by enzyme-linked immunostaining of the erythrocyte membrane, using alkaline phosphatase a...