Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene
The recent resurgence of Plasmodium vivax malaria requires close epidemiological surveillance and monitoring of the circulating parasite populations. In this study, we developed a combination of polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP) method to investigate t...
Saved in:
Main Authors: | , , , , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2018
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/23282 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.23282 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.232822018-08-20T14:07:03Z Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene Zhaoqing Yang Jun Miao Yaming Huang Xinyi Li Chaturong Putaporntip Somchai Jongwutiwes Qi Gao Rachanee Udomsangpetch Jetsumon Sattabongkot Liwang Cui Kunming Medical College Malaria Department Chulalongkorn University Jiangsu Institute of Parasitic Diseases Mahidol University Armed Forces Research Institute of Medical Sciences, Thailand Pennsylvania State University Immunology and Microbiology Medicine The recent resurgence of Plasmodium vivax malaria requires close epidemiological surveillance and monitoring of the circulating parasite populations. In this study, we developed a combination of polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP) method to investigate the genetic diversity of the P. vivax merozoite surface protein 3β (PvMSP3β) gene among four Asian parasite populations representing both tropical and temperate strains with dramatic divergent relapse patterns (N = 143). Using P. vivax field isolates from symptomatic patients, we have validated the feasibility of this protocol in distinguishing parasite genotypes. We have shown that PCR alone could detect three major size polymorphisms of the PvMSP3β gene, and restriction analysis detected a total of 12 alleles within these Asian samples. Samples from different geographical areas differed dramatically in their PvMSP3β allele composition and frequency, indicating that complex, yet different parasite genotypes were circulating in different endemic areas. This protocol allowed easy detections of multiple infections, which reached 20.5% in the samples from Thailand. It is interesting to note that samples from one temperate site in China collected during a recent outbreak of the disease also showed a high level of genetic diversity with multiple infections accounting for 5.6% of the samples. When combined with the PvMSP3α locus, this method provides better capability in distinguishing P. vivax genotypes and detecting mixed genotype infections. © 2006 Elsevier B.V. All rights reserved. 2018-08-20T06:59:50Z 2018-08-20T06:59:50Z 2006-12-01 Article Acta Tropica. Vol.100, No.3 (2006), 205-212 10.1016/j.actatropica.2006.10.011 0001706X 2-s2.0-33846581910 https://repository.li.mahidol.ac.th/handle/123456789/23282 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33846581910&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Immunology and Microbiology Medicine |
spellingShingle |
Immunology and Microbiology Medicine Zhaoqing Yang Jun Miao Yaming Huang Xinyi Li Chaturong Putaporntip Somchai Jongwutiwes Qi Gao Rachanee Udomsangpetch Jetsumon Sattabongkot Liwang Cui Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene |
description |
The recent resurgence of Plasmodium vivax malaria requires close epidemiological surveillance and monitoring of the circulating parasite populations. In this study, we developed a combination of polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP) method to investigate the genetic diversity of the P. vivax merozoite surface protein 3β (PvMSP3β) gene among four Asian parasite populations representing both tropical and temperate strains with dramatic divergent relapse patterns (N = 143). Using P. vivax field isolates from symptomatic patients, we have validated the feasibility of this protocol in distinguishing parasite genotypes. We have shown that PCR alone could detect three major size polymorphisms of the PvMSP3β gene, and restriction analysis detected a total of 12 alleles within these Asian samples. Samples from different geographical areas differed dramatically in their PvMSP3β allele composition and frequency, indicating that complex, yet different parasite genotypes were circulating in different endemic areas. This protocol allowed easy detections of multiple infections, which reached 20.5% in the samples from Thailand. It is interesting to note that samples from one temperate site in China collected during a recent outbreak of the disease also showed a high level of genetic diversity with multiple infections accounting for 5.6% of the samples. When combined with the PvMSP3α locus, this method provides better capability in distinguishing P. vivax genotypes and detecting mixed genotype infections. © 2006 Elsevier B.V. All rights reserved. |
author2 |
Kunming Medical College |
author_facet |
Kunming Medical College Zhaoqing Yang Jun Miao Yaming Huang Xinyi Li Chaturong Putaporntip Somchai Jongwutiwes Qi Gao Rachanee Udomsangpetch Jetsumon Sattabongkot Liwang Cui |
format |
Article |
author |
Zhaoqing Yang Jun Miao Yaming Huang Xinyi Li Chaturong Putaporntip Somchai Jongwutiwes Qi Gao Rachanee Udomsangpetch Jetsumon Sattabongkot Liwang Cui |
author_sort |
Zhaoqing Yang |
title |
Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene |
title_short |
Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene |
title_full |
Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene |
title_fullStr |
Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene |
title_full_unstemmed |
Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene |
title_sort |
genetic structures of geographically distinct plasmodium vivax populations assessed by pcr/rflp analysis of the merozoite surface protein 3β gene |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/23282 |
_version_ |
1763494162460573696 |