In vitro interaction between artemisinin and chloroquine as well as desbutyl-benflumetol in Plasmodium vivax
Malaria resulting from infection with Plas-modium vivax rarely causes death, however, patients usually suffer acute debilitating clinical symptoms and the recovery is slow. This study had the objective of assessing the pharmacodynamic interaction between artimisinin and chloroquine with a view of a...
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th-mahidol.234472018-08-20T14:06:07Z In vitro interaction between artemisinin and chloroquine as well as desbutyl-benflumetol in Plasmodium vivax Leila Kyavar Chaiporn Rojanawatsirivet Herwig Kollaritsch Gunther Wernsdorfer Jeeraphat Sirichaisinthop Walther H. Wernsdorfer Medizinische Universitat Wien Thailand Ministry of Public Health Mahidol University Medicine Malaria resulting from infection with Plas-modium vivax rarely causes death, however, patients usually suffer acute debilitating clinical symptoms and the recovery is slow. This study had the objective of assessing the pharmacodynamic interaction between artimisinin and chloroquine with a view of a potential acceleration of the clinical-parasitological response, and the investigation of therapeutic alternatives in the event of chloroquine resistance in Plasmodium vivax. Tests were based on the growth inhibition of Plasmodium vivax, determined by morphological differential counts of 200 asexual parasites. In total 45 isolates were evaluated successfully with parallel tests for artemisinin, chloroquine and desbutyl-benflumetol (DBB) alone and combinations of artemisinin + chloroquine and artemisinin + DBB. Total inhibition was reached at a mean concentration of 1274.8 nM (95% CI 898.5 to 1808.7 nM), and 1852.2 nM (95% CI 1539.5 to 2228.6 nM) for artemisinin, and chloroquine respectively, whilst the 1:1 (m/m) combination of artemisinin and chloroquine was 1860.2 nM (95% CI 1454.4 to 2379.3 nM). EC50and EC90were 129.9 nM and 1058.5 nM for chloroquine, 32.6 nM and 735.5 nM for artemisinin, and 73.6 nM and 1103.0 nM for the 1:1 combination of both drugs. Interaction analysis according to Berenbaum yielded for the artemisinin + chloroquine combination at the EC50a mean ∑FIC of 1.1126, at the EC90a mean ∑FIC of 1.0331, and at the EC99a mean ∑FIC of 1.1857. These results revealed marked additive interaction. For desbutyl-benflumetol (DBB) the EC50and EC90were 1.5 nM and 28.8 nM, complete growth inhibition was observed at 90.4 nM (95% CI 75.1 to 108.7 nM). Interaction analysis indicated moderate antagonism at the lower concentration ranges, at the EC90additive interaction with a mean ∑FIC of 1.0300, and synergism at the therapeutically most important EC99with a mean ∑FIC of 0.5990. © Springer-Verlag 2006. 2018-08-20T07:06:07Z 2018-08-20T07:06:07Z 2006-12-01 Article Wiener Klinische Wochenschrift, Supplement. Vol.118, No.3 (2006), 62-69 10.1007/s00508-006-0677-z 03005178 2-s2.0-33846391019 https://repository.li.mahidol.ac.th/handle/123456789/23447 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33846391019&origin=inward |
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Medicine Leila Kyavar Chaiporn Rojanawatsirivet Herwig Kollaritsch Gunther Wernsdorfer Jeeraphat Sirichaisinthop Walther H. Wernsdorfer In vitro interaction between artemisinin and chloroquine as well as desbutyl-benflumetol in Plasmodium vivax |
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Malaria resulting from infection with Plas-modium vivax rarely causes death, however, patients usually suffer acute debilitating clinical symptoms and the recovery is slow. This study had the objective of assessing the pharmacodynamic interaction between artimisinin and chloroquine with a view of a potential acceleration of the clinical-parasitological response, and the investigation of therapeutic alternatives in the event of chloroquine resistance in Plasmodium vivax. Tests were based on the growth inhibition of Plasmodium vivax, determined by morphological differential counts of 200 asexual parasites. In total 45 isolates were evaluated successfully with parallel tests for artemisinin, chloroquine and desbutyl-benflumetol (DBB) alone and combinations of artemisinin + chloroquine and artemisinin + DBB. Total inhibition was reached at a mean concentration of 1274.8 nM (95% CI 898.5 to 1808.7 nM), and 1852.2 nM (95% CI 1539.5 to 2228.6 nM) for artemisinin, and chloroquine respectively, whilst the 1:1 (m/m) combination of artemisinin and chloroquine was 1860.2 nM (95% CI 1454.4 to 2379.3 nM). EC50and EC90were 129.9 nM and 1058.5 nM for chloroquine, 32.6 nM and 735.5 nM for artemisinin, and 73.6 nM and 1103.0 nM for the 1:1 combination of both drugs. Interaction analysis according to Berenbaum yielded for the artemisinin + chloroquine combination at the EC50a mean ∑FIC of 1.1126, at the EC90a mean ∑FIC of 1.0331, and at the EC99a mean ∑FIC of 1.1857. These results revealed marked additive interaction. For desbutyl-benflumetol (DBB) the EC50and EC90were 1.5 nM and 28.8 nM, complete growth inhibition was observed at 90.4 nM (95% CI 75.1 to 108.7 nM). Interaction analysis indicated moderate antagonism at the lower concentration ranges, at the EC90additive interaction with a mean ∑FIC of 1.0300, and synergism at the therapeutically most important EC99with a mean ∑FIC of 0.5990. © Springer-Verlag 2006. |
author2 |
Medizinische Universitat Wien |
author_facet |
Medizinische Universitat Wien Leila Kyavar Chaiporn Rojanawatsirivet Herwig Kollaritsch Gunther Wernsdorfer Jeeraphat Sirichaisinthop Walther H. Wernsdorfer |
format |
Article |
author |
Leila Kyavar Chaiporn Rojanawatsirivet Herwig Kollaritsch Gunther Wernsdorfer Jeeraphat Sirichaisinthop Walther H. Wernsdorfer |
author_sort |
Leila Kyavar |
title |
In vitro interaction between artemisinin and chloroquine as well as desbutyl-benflumetol in Plasmodium vivax |
title_short |
In vitro interaction between artemisinin and chloroquine as well as desbutyl-benflumetol in Plasmodium vivax |
title_full |
In vitro interaction between artemisinin and chloroquine as well as desbutyl-benflumetol in Plasmodium vivax |
title_fullStr |
In vitro interaction between artemisinin and chloroquine as well as desbutyl-benflumetol in Plasmodium vivax |
title_full_unstemmed |
In vitro interaction between artemisinin and chloroquine as well as desbutyl-benflumetol in Plasmodium vivax |
title_sort |
in vitro interaction between artemisinin and chloroquine as well as desbutyl-benflumetol in plasmodium vivax |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/23447 |
_version_ |
1763496812680839168 |