Conserved Glu40 and Glu433 of the biotin carboxylase domain of yeast pyruvate carboxylase I isoenzyme are essential for the association of tetramers

• Main Authors: Sarawut Jitrapakdee, Katharina H. Surinya, Abdussalam Adina-Zada, Steven W. Polyak, Cvetan Stojkoski, Roger Smyth, Grant W. Booker, W. Wallace Cleland, Paul V. Attwood, John C. Wallace
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/24119

The native form of pyruvate carboxylase is an α4tetramer but the tetramerisation domain of each subunit is currently unknown. To identify this domain we co-expressed yeast pyruvate carboxylase 1 isozyme (Pyc1) with an N-terminal myc tag, together with constructs encoding either the biotin carboxylas...