Evaluation of HIV-1 viral load detection by modified a commercial real-time PCR reagent used for light cycler 1.2 instrument
Objective: Commercial TaqMan real-time PCR reagent was modified and applied on Light Cylcer 1.2 for quantifying HIV-1 RNA in plasma and compared with the reference method; COBAS AmpliPrep/COBAS Amplicor HIV-1 monitor test version 1.5. Material and Method: Three hundred and eight frozen and fresh pla...
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th-mahidol.246962018-08-24T08:59:17Z Evaluation of HIV-1 viral load detection by modified a commercial real-time PCR reagent used for light cycler 1.2 instrument Sumonmal Uttayamakul Sirirat Likanonsakul Ruengpung Sutthent Achara Chaovavanich Bamrasnaradura Infectious Disease Institute Mahidol University Medicine Objective: Commercial TaqMan real-time PCR reagent was modified and applied on Light Cylcer 1.2 for quantifying HIV-1 RNA in plasma and compared with the reference method; COBAS AmpliPrep/COBAS Amplicor HIV-1 monitor test version 1.5. Material and Method: Three hundred and eight frozen and fresh plasma samples were used for evaluation. Sequential specimens were also tested for follow-up cases. Results: The correlation between HIV-1 RNA values obtained by reference and modified method with automated and manual sample preparation were significant with r = 0.916 and 0.908 (p < 0.001, p < 0.001) respectively with similar agreement log of mean bias (0.5 versus 0.48). High degree of correlation and agreement were observed between the assays in blind fresh plasma, r = 0.953 (p < 0.001) with 0.15 log difference in HIV-1 RNA level. Among follow-up samples, both methods gave 100% concordant results. Conclusion: This modified protocol provided evidence for using modified commercial real-time PCR reagent for HIV-1 RNA quantitative detection as a monitoring tool for HIV/AIDS patients in Thailand. 2018-08-24T01:59:17Z 2018-08-24T01:59:17Z 2007-11-01 Article Journal of the Medical Association of Thailand. Vol.90, No.11 (2007), 2429-2436 01252208 01252208 2-s2.0-37148999653 https://repository.li.mahidol.ac.th/handle/123456789/24696 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=37148999653&origin=inward |
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Medicine Sumonmal Uttayamakul Sirirat Likanonsakul Ruengpung Sutthent Achara Chaovavanich Evaluation of HIV-1 viral load detection by modified a commercial real-time PCR reagent used for light cycler 1.2 instrument |
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Objective: Commercial TaqMan real-time PCR reagent was modified and applied on Light Cylcer 1.2 for quantifying HIV-1 RNA in plasma and compared with the reference method; COBAS AmpliPrep/COBAS Amplicor HIV-1 monitor test version 1.5. Material and Method: Three hundred and eight frozen and fresh plasma samples were used for evaluation. Sequential specimens were also tested for follow-up cases. Results: The correlation between HIV-1 RNA values obtained by reference and modified method with automated and manual sample preparation were significant with r = 0.916 and 0.908 (p < 0.001, p < 0.001) respectively with similar agreement log of mean bias (0.5 versus 0.48). High degree of correlation and agreement were observed between the assays in blind fresh plasma, r = 0.953 (p < 0.001) with 0.15 log difference in HIV-1 RNA level. Among follow-up samples, both methods gave 100% concordant results. Conclusion: This modified protocol provided evidence for using modified commercial real-time PCR reagent for HIV-1 RNA quantitative detection as a monitoring tool for HIV/AIDS patients in Thailand. |
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Bamrasnaradura Infectious Disease Institute |
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Bamrasnaradura Infectious Disease Institute Sumonmal Uttayamakul Sirirat Likanonsakul Ruengpung Sutthent Achara Chaovavanich |
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Article |
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Sumonmal Uttayamakul Sirirat Likanonsakul Ruengpung Sutthent Achara Chaovavanich |
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Sumonmal Uttayamakul |
title |
Evaluation of HIV-1 viral load detection by modified a commercial real-time PCR reagent used for light cycler 1.2 instrument |
title_short |
Evaluation of HIV-1 viral load detection by modified a commercial real-time PCR reagent used for light cycler 1.2 instrument |
title_full |
Evaluation of HIV-1 viral load detection by modified a commercial real-time PCR reagent used for light cycler 1.2 instrument |
title_fullStr |
Evaluation of HIV-1 viral load detection by modified a commercial real-time PCR reagent used for light cycler 1.2 instrument |
title_full_unstemmed |
Evaluation of HIV-1 viral load detection by modified a commercial real-time PCR reagent used for light cycler 1.2 instrument |
title_sort |
evaluation of hiv-1 viral load detection by modified a commercial real-time pcr reagent used for light cycler 1.2 instrument |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/24696 |
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1763493786124550144 |