Patient and sample-related factors that effect the success of in vitro isolation of Orientia tsutsugamushi
Orientia tsutsugamushi is the causative agent of scrub typhus infection, a major cause of human disease in rural areas of Southeast Asia. Twenty-six blood samples collected from patients with serologically proven scrub typhus during a six month period were sent to Bangkok (535 km from the clinical s...
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th-mahidol.250752018-08-24T09:12:51Z Patient and sample-related factors that effect the success of in vitro isolation of Orientia tsutsugamushi Rungnapa Luksameetanasan Stuart D. Blacksell Thareerat Kalambaheti Vanaporn Wuthiekanun Wirongrong Chierakul Sunee Chueasuwanchai Apichat Apiwattanaporn John Stenos Stephen Graves Sharon J. Peacock N. P J Day Mahidol University John Radcliffe Hospital Udon Thani Center Hospital Geelong Hospital Medicine Orientia tsutsugamushi is the causative agent of scrub typhus infection, a major cause of human disease in rural areas of Southeast Asia. Twenty-six blood samples collected from patients with serologically proven scrub typhus during a six month period were sent to Bangkok (535 km from the clinical site) by road at ambient temperature (average daily temperature range: 27.1-29.1°C) for attempted in vitro isolation in Vero cells. O. tsutsugamushi was isolated from 12 samples (sensitivity 46.7%) with the time to isolation ranging from 16 to 37 days [median 27 days, inter-quartile range (IQR) 22.5-33.5 days]. Patient factors such as days of fever and O. tsutsugamushi IgM antibody titer, transport factors such as transit time, and isolate genotype (Karp and Gilliam/Kawasaki) were assessed to determine their influence on the outcome of in vitro isolation. None of the factors significantly influenced the isolation outcome. This study demonstrates that O. tsutsugamushi can often be isolated in vitro from the blood of scrub typhus patients when transported at ambient tropical temperatures for many days. 2018-08-24T02:12:51Z 2018-08-24T02:12:51Z 2007-01-01 Article Southeast Asian Journal of Tropical Medicine and Public Health. Vol.38, No.1 (2007), 91-96 01251562 2-s2.0-33947599058 https://repository.li.mahidol.ac.th/handle/123456789/25075 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33947599058&origin=inward |
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Medicine Rungnapa Luksameetanasan Stuart D. Blacksell Thareerat Kalambaheti Vanaporn Wuthiekanun Wirongrong Chierakul Sunee Chueasuwanchai Apichat Apiwattanaporn John Stenos Stephen Graves Sharon J. Peacock N. P J Day Patient and sample-related factors that effect the success of in vitro isolation of Orientia tsutsugamushi |
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Orientia tsutsugamushi is the causative agent of scrub typhus infection, a major cause of human disease in rural areas of Southeast Asia. Twenty-six blood samples collected from patients with serologically proven scrub typhus during a six month period were sent to Bangkok (535 km from the clinical site) by road at ambient temperature (average daily temperature range: 27.1-29.1°C) for attempted in vitro isolation in Vero cells. O. tsutsugamushi was isolated from 12 samples (sensitivity 46.7%) with the time to isolation ranging from 16 to 37 days [median 27 days, inter-quartile range (IQR) 22.5-33.5 days]. Patient factors such as days of fever and O. tsutsugamushi IgM antibody titer, transport factors such as transit time, and isolate genotype (Karp and Gilliam/Kawasaki) were assessed to determine their influence on the outcome of in vitro isolation. None of the factors significantly influenced the isolation outcome. This study demonstrates that O. tsutsugamushi can often be isolated in vitro from the blood of scrub typhus patients when transported at ambient tropical temperatures for many days. |
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Mahidol University |
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Mahidol University Rungnapa Luksameetanasan Stuart D. Blacksell Thareerat Kalambaheti Vanaporn Wuthiekanun Wirongrong Chierakul Sunee Chueasuwanchai Apichat Apiwattanaporn John Stenos Stephen Graves Sharon J. Peacock N. P J Day |
format |
Article |
author |
Rungnapa Luksameetanasan Stuart D. Blacksell Thareerat Kalambaheti Vanaporn Wuthiekanun Wirongrong Chierakul Sunee Chueasuwanchai Apichat Apiwattanaporn John Stenos Stephen Graves Sharon J. Peacock N. P J Day |
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Rungnapa Luksameetanasan |
title |
Patient and sample-related factors that effect the success of in vitro isolation of Orientia tsutsugamushi |
title_short |
Patient and sample-related factors that effect the success of in vitro isolation of Orientia tsutsugamushi |
title_full |
Patient and sample-related factors that effect the success of in vitro isolation of Orientia tsutsugamushi |
title_fullStr |
Patient and sample-related factors that effect the success of in vitro isolation of Orientia tsutsugamushi |
title_full_unstemmed |
Patient and sample-related factors that effect the success of in vitro isolation of Orientia tsutsugamushi |
title_sort |
patient and sample-related factors that effect the success of in vitro isolation of orientia tsutsugamushi |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/25075 |
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1763492479418499072 |