Expression and Biochemical Characterization of the Bacillus thuringiensis Cry4B α1-α5 Pore-forming Fragment

• Main Authors: Theeraporn Puntheeranurak, Somphob Leetacheewa, Gerd Katzenmeier, Chartchai Krittanai, Sakol Panyim, Chanan Angsuthanasombat
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/26456

Tryptic activation of the 130-kDa Bacillus thuringiensis Cry4B δ-endotoxin produced protease-resistant products of ca. 47 kDa and ca. 21 kDa. The 21-kDa fragment was identified as the N-terminal five-helix bundle (α1-α5), which is a potential candidate for membrane insertion and pore formation. In t...