Limited global diversity of the Plasmodium vivax merozoite surface protein 4 gene
Merozoite surface proteins (MSPs) of the malaria parasites are major candidates for vaccine development targeting asexual blood stages. However, the diverse antigenic repertoire of these antigens that induce strain-specific protective immunity in human is a major challenge for vaccine design and oft...
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th-mahidol.269852018-09-13T13:56:03Z Limited global diversity of the Plasmodium vivax merozoite surface protein 4 gene Chaturong Putaporntip Somchai Jongwutiwes Marcelo U. Ferreira Hiroji Kanbara Rachanee Udomsangpetch Liwang Cui Chulalongkorn University Universidade de Sao Paulo - USP Nagasaki University Mahidol University Pennsylvania State University Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine Merozoite surface proteins (MSPs) of the malaria parasites are major candidates for vaccine development targeting asexual blood stages. However, the diverse antigenic repertoire of these antigens that induce strain-specific protective immunity in human is a major challenge for vaccine design and often determines the efficacy of a vaccine. Here we further assessed the genetic diversity of Plasmodium vivax MSP4 (PvMSP4) protein using 195 parasite samples collected mostly from Thailand, Indonesia and Brazil. Overall, PvMSP4 is highly conserved with only eight amino acid substitutions. The majority of the haplotype diversity was restricted to the two short tetrapeptide repeat arrays in exon 1 and 2, respectively. Selection and neutrality tests indicated that exon 1 and the entire coding region of PvMSP4 were under purifying selection. Despite the limited nucleotide polymorphism of PvMSP4, significant genetic differentiation among the three major parasite populations was detected. Moreover, microgeographical heterogeneity was also evident in the parasite populations from different endemic areas of Thailand. © 2009 Elsevier B.V. All rights reserved. 2018-09-13T06:17:56Z 2018-09-13T06:17:56Z 2009-09-01 Article Infection, Genetics and Evolution. Vol.9, No.5 (2009), 821-826 10.1016/j.meegid.2009.04.017 15671348 2-s2.0-67749132432 https://repository.li.mahidol.ac.th/handle/123456789/26985 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67749132432&origin=inward |
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Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine Chaturong Putaporntip Somchai Jongwutiwes Marcelo U. Ferreira Hiroji Kanbara Rachanee Udomsangpetch Liwang Cui Limited global diversity of the Plasmodium vivax merozoite surface protein 4 gene |
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Merozoite surface proteins (MSPs) of the malaria parasites are major candidates for vaccine development targeting asexual blood stages. However, the diverse antigenic repertoire of these antigens that induce strain-specific protective immunity in human is a major challenge for vaccine design and often determines the efficacy of a vaccine. Here we further assessed the genetic diversity of Plasmodium vivax MSP4 (PvMSP4) protein using 195 parasite samples collected mostly from Thailand, Indonesia and Brazil. Overall, PvMSP4 is highly conserved with only eight amino acid substitutions. The majority of the haplotype diversity was restricted to the two short tetrapeptide repeat arrays in exon 1 and 2, respectively. Selection and neutrality tests indicated that exon 1 and the entire coding region of PvMSP4 were under purifying selection. Despite the limited nucleotide polymorphism of PvMSP4, significant genetic differentiation among the three major parasite populations was detected. Moreover, microgeographical heterogeneity was also evident in the parasite populations from different endemic areas of Thailand. © 2009 Elsevier B.V. All rights reserved. |
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Chulalongkorn University |
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Chulalongkorn University Chaturong Putaporntip Somchai Jongwutiwes Marcelo U. Ferreira Hiroji Kanbara Rachanee Udomsangpetch Liwang Cui |
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Article |
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Chaturong Putaporntip Somchai Jongwutiwes Marcelo U. Ferreira Hiroji Kanbara Rachanee Udomsangpetch Liwang Cui |
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Chaturong Putaporntip |
title |
Limited global diversity of the Plasmodium vivax merozoite surface protein 4 gene |
title_short |
Limited global diversity of the Plasmodium vivax merozoite surface protein 4 gene |
title_full |
Limited global diversity of the Plasmodium vivax merozoite surface protein 4 gene |
title_fullStr |
Limited global diversity of the Plasmodium vivax merozoite surface protein 4 gene |
title_full_unstemmed |
Limited global diversity of the Plasmodium vivax merozoite surface protein 4 gene |
title_sort |
limited global diversity of the plasmodium vivax merozoite surface protein 4 gene |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/26985 |
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1763488676460888064 |