Crystallization and preliminary crystallographic studies of dihydrofolate reductase-thymidylate synthase from Trypanosoma cruzi, the Chagas disease pathogen

• Main Authors: Penchit Chitnumsub, Jirundon Yuvaniyama, Thippayarat Chahomchuen, Tirayut Vilaivan, Yongyuth Yuthavong
• Other Authors: Thailand National Center for Genetic Engineering and Biotechnology
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Physics and Astronomy
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/27110
• Institution: Mahidol University

Trypanosoma cruzi dihydrofolate reductase-thymidylate synthase (TcDHFR-TS) was crystallized in complexes with the dihydrotriazine-based or quinazoline-based antifolates C-448, cycloguanil (CYC) and Q-8 in order to gain insight into the interactions of this DHFR enzyme with classical and novel inhibi...