Human monoclonal ScFv neutralize lethal Thai cobra, Naja kaouthia, neurotoxin
Animal derived anti-Naja. kaouthia (Thai cobra) venom is used for specific treatment of the snake bitten victims. Many recipients develop allergic reaction or anti-isotype response which causes serum sickness. A better therapeutic antibody is needed. In this study, long α-neurotoxin was purified fro...
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th-mahidol.272682018-09-13T13:26:10Z Human monoclonal ScFv neutralize lethal Thai cobra, Naja kaouthia, neurotoxin Kasem Kulkeaw Yuwaporn Sakolvaree Potjanee Srimanote Pongsri Tongtawe Santi Maneewatch Nitat Sookrung Anchalee Tungtrongchitr Pramuan Tapchaisri Hisao Kurazono Wanpen Chaicumpa Thammasat University Mahidol University Obihiro University of Agriculture and Veterinary Medicine Biochemistry, Genetics and Molecular Biology Animal derived anti-Naja. kaouthia (Thai cobra) venom is used for specific treatment of the snake bitten victims. Many recipients develop allergic reaction or anti-isotype response which causes serum sickness. A better therapeutic antibody is needed. In this study, long α-neurotoxin was purified from the N. kaouthia holovenom and verified by 2D-LC/MS-MS. The toxin was used as antigen in a phage bio-panning to select phage clones displaying human single chain variable antibody fragments (HuScFv) from a phage display antibody library constructed from immunoglobulin genes of non-immunized Thai blood donors. HuScFv that specifically bound to the neurotoxin were produced from huscfv-phagemid transformed E. coli clones and affinity purified. The HuScFv could neutralize toxicity of the N. kaouthia neurotoxin and rescued the envenomized mice from the neurotoxin mediated lethality. Peptide mimotope of the neutralizing HuScFv matched with an amino acid sequence (epitope) located in the loop-3 of the N. kaouthia long α-neurotoxin which functions in acetylcholine receptor binding. The mimotope is also similar to peptide sequences found on other snake venom neurotoxins implying a possibility of the HuScFv to exert pan-neutralizing activity against multiple snake neurotoxins. © 2009 Elsevier B.V. All rights reserved. 2018-09-13T06:26:10Z 2018-09-13T06:26:10Z 2009-03-06 Article Journal of Proteomics. Vol.72, No.2 (2009), 270-282 10.1016/j.jprot.2008.12.007 18743919 2-s2.0-60149088262 https://repository.li.mahidol.ac.th/handle/123456789/27268 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=60149088262&origin=inward |
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Biochemistry, Genetics and Molecular Biology Kasem Kulkeaw Yuwaporn Sakolvaree Potjanee Srimanote Pongsri Tongtawe Santi Maneewatch Nitat Sookrung Anchalee Tungtrongchitr Pramuan Tapchaisri Hisao Kurazono Wanpen Chaicumpa Human monoclonal ScFv neutralize lethal Thai cobra, Naja kaouthia, neurotoxin |
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Animal derived anti-Naja. kaouthia (Thai cobra) venom is used for specific treatment of the snake bitten victims. Many recipients develop allergic reaction or anti-isotype response which causes serum sickness. A better therapeutic antibody is needed. In this study, long α-neurotoxin was purified from the N. kaouthia holovenom and verified by 2D-LC/MS-MS. The toxin was used as antigen in a phage bio-panning to select phage clones displaying human single chain variable antibody fragments (HuScFv) from a phage display antibody library constructed from immunoglobulin genes of non-immunized Thai blood donors. HuScFv that specifically bound to the neurotoxin were produced from huscfv-phagemid transformed E. coli clones and affinity purified. The HuScFv could neutralize toxicity of the N. kaouthia neurotoxin and rescued the envenomized mice from the neurotoxin mediated lethality. Peptide mimotope of the neutralizing HuScFv matched with an amino acid sequence (epitope) located in the loop-3 of the N. kaouthia long α-neurotoxin which functions in acetylcholine receptor binding. The mimotope is also similar to peptide sequences found on other snake venom neurotoxins implying a possibility of the HuScFv to exert pan-neutralizing activity against multiple snake neurotoxins. © 2009 Elsevier B.V. All rights reserved. |
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Thammasat University |
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Thammasat University Kasem Kulkeaw Yuwaporn Sakolvaree Potjanee Srimanote Pongsri Tongtawe Santi Maneewatch Nitat Sookrung Anchalee Tungtrongchitr Pramuan Tapchaisri Hisao Kurazono Wanpen Chaicumpa |
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Article |
author |
Kasem Kulkeaw Yuwaporn Sakolvaree Potjanee Srimanote Pongsri Tongtawe Santi Maneewatch Nitat Sookrung Anchalee Tungtrongchitr Pramuan Tapchaisri Hisao Kurazono Wanpen Chaicumpa |
author_sort |
Kasem Kulkeaw |
title |
Human monoclonal ScFv neutralize lethal Thai cobra, Naja kaouthia, neurotoxin |
title_short |
Human monoclonal ScFv neutralize lethal Thai cobra, Naja kaouthia, neurotoxin |
title_full |
Human monoclonal ScFv neutralize lethal Thai cobra, Naja kaouthia, neurotoxin |
title_fullStr |
Human monoclonal ScFv neutralize lethal Thai cobra, Naja kaouthia, neurotoxin |
title_full_unstemmed |
Human monoclonal ScFv neutralize lethal Thai cobra, Naja kaouthia, neurotoxin |
title_sort |
human monoclonal scfv neutralize lethal thai cobra, naja kaouthia, neurotoxin |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/27268 |
_version_ |
1763493830771867648 |