Effective and cheap removal of leukocytes and platelets from plasmodium vivax infected blood
Background. Investigations of Plasmodium vivax are restricted to samples collected from infected persons or primates, because this parasite cannot be maintained in in vitro cultures. Contamination of P. vivax isolates with host leukocytes and platelets is detrimental to a range of ex vivo and molecu...
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th-mahidol.276882018-09-13T13:57:45Z Effective and cheap removal of leukocytes and platelets from plasmodium vivax infected blood Kanlaya Sriprawat Supaporn Kaewpongsri Rossarin Suwanarusk Mara L. Leimanis Usa Lek-Uthai Aung Pyae Phyo Georges Snounou Bruce Russell Laurent Renia François Nosten Shoklo Malaria Research Unit Agency for Science, Technology and Research, Singapore Mahidol University Hopital Pitie Salpetriere Faculte de Medecine Pierre et Marie Curie National University of Singapore, Faculty of Medicine National University of Singapore Nuffield Department of Clinical Medicine Immunology and Microbiology Medicine Background. Investigations of Plasmodium vivax are restricted to samples collected from infected persons or primates, because this parasite cannot be maintained in in vitro cultures. Contamination of P. vivax isolates with host leukocytes and platelets is detrimental to a range of ex vivo and molecular investigations. Easy-to-produce CF11 cellulose filters have recently provided us with an inexpensive method for the removal of leukocytes and platelets. This contrasted with previous reports of unacceptably high levels of infected red blood cell (IRBC) retention by CF11. The aims of this study were to compare the ability of CF11 cellulose filters and the commercial filter Plasmodipur at removing leukocyte and platelet, and to investigate the retention of P. vivax IRBCs by CF11 cellulose filtration. Methods and Results. Side-by-side comparison of six leukocyte removal methods using blood samples from five healthy donor showed that CF11 filtration reduced the mean initial leukocyte counts from 9.4 × 103per μl [95%CI 5.213.5] to 0.01 × 103[95%CI 0.010.03]. The CF11 was particularly effective at removing neutrophils. CF11 treatment also reduced initial platelet counts from 211.6 × 103per μl[95%CI 107.5315.7] to 0.8 × 103per μl [95%CI -0.72.2]. Analysis of 30 P. vivax blood samples before and after CF11 filtration showed only a minor loss in parasitaemia ( ≤7.1% of initial counts). Stage specific retention of P. vivax IRBCs was not observed. Conclusion. CF11 filtration is the most cost and time efficient method for the production of leukocyte- and platelet-free P. vivax-infected erythrocytes from field isolates. © 2009 Sriprawat et al.; licensee BioMed Central Ltd. 2018-09-13T06:42:12Z 2018-09-13T06:42:12Z 2009-07-23 Article Malaria Journal. Vol.8, No.1 (2009) 10.1186/1475-2875-8-115 14752875 2-s2.0-67650658803 https://repository.li.mahidol.ac.th/handle/123456789/27688 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67650658803&origin=inward |
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Immunology and Microbiology Medicine Kanlaya Sriprawat Supaporn Kaewpongsri Rossarin Suwanarusk Mara L. Leimanis Usa Lek-Uthai Aung Pyae Phyo Georges Snounou Bruce Russell Laurent Renia François Nosten Effective and cheap removal of leukocytes and platelets from plasmodium vivax infected blood |
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Background. Investigations of Plasmodium vivax are restricted to samples collected from infected persons or primates, because this parasite cannot be maintained in in vitro cultures. Contamination of P. vivax isolates with host leukocytes and platelets is detrimental to a range of ex vivo and molecular investigations. Easy-to-produce CF11 cellulose filters have recently provided us with an inexpensive method for the removal of leukocytes and platelets. This contrasted with previous reports of unacceptably high levels of infected red blood cell (IRBC) retention by CF11. The aims of this study were to compare the ability of CF11 cellulose filters and the commercial filter Plasmodipur at removing leukocyte and platelet, and to investigate the retention of P. vivax IRBCs by CF11 cellulose filtration. Methods and Results. Side-by-side comparison of six leukocyte removal methods using blood samples from five healthy donor showed that CF11 filtration reduced the mean initial leukocyte counts from 9.4 × 103per μl [95%CI 5.213.5] to 0.01 × 103[95%CI 0.010.03]. The CF11 was particularly effective at removing neutrophils. CF11 treatment also reduced initial platelet counts from 211.6 × 103per μl[95%CI 107.5315.7] to 0.8 × 103per μl [95%CI -0.72.2]. Analysis of 30 P. vivax blood samples before and after CF11 filtration showed only a minor loss in parasitaemia ( ≤7.1% of initial counts). Stage specific retention of P. vivax IRBCs was not observed. Conclusion. CF11 filtration is the most cost and time efficient method for the production of leukocyte- and platelet-free P. vivax-infected erythrocytes from field isolates. © 2009 Sriprawat et al.; licensee BioMed Central Ltd. |
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Shoklo Malaria Research Unit |
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Shoklo Malaria Research Unit Kanlaya Sriprawat Supaporn Kaewpongsri Rossarin Suwanarusk Mara L. Leimanis Usa Lek-Uthai Aung Pyae Phyo Georges Snounou Bruce Russell Laurent Renia François Nosten |
format |
Article |
author |
Kanlaya Sriprawat Supaporn Kaewpongsri Rossarin Suwanarusk Mara L. Leimanis Usa Lek-Uthai Aung Pyae Phyo Georges Snounou Bruce Russell Laurent Renia François Nosten |
author_sort |
Kanlaya Sriprawat |
title |
Effective and cheap removal of leukocytes and platelets from plasmodium vivax infected blood |
title_short |
Effective and cheap removal of leukocytes and platelets from plasmodium vivax infected blood |
title_full |
Effective and cheap removal of leukocytes and platelets from plasmodium vivax infected blood |
title_fullStr |
Effective and cheap removal of leukocytes and platelets from plasmodium vivax infected blood |
title_full_unstemmed |
Effective and cheap removal of leukocytes and platelets from plasmodium vivax infected blood |
title_sort |
effective and cheap removal of leukocytes and platelets from plasmodium vivax infected blood |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/27688 |
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1763487362871984128 |