Differential prevalence of plasmodium infections and cryptic plasmodium knowlesi malaria in humans in Thailand
Background. A case of human infection with Plasmodium knowlesi has been recently discovered in Thailand. To investigate the prevalence of this malaria species, a molecular-based survey was performed. Methods. Blood samples from 1874 patients were tested for Plasmodium species by microscopy and neste...
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th-mahidol.281152018-09-13T14:02:27Z Differential prevalence of plasmodium infections and cryptic plasmodium knowlesi malaria in humans in Thailand Chaturong Putaporntip Thongchai Hongsrimuang Sunee Seethamchai Teerayot Kobasa Kriengsak Limkittikul Liwang Cui Somchai Jongwutiwes Molecular Biology of Malaria and Opportunistic Parasites Research Unit Mahidol University Naresuan University Thailand Ministry of Public Health Pennsylvania State University Chulalongkorn University Medicine Background. A case of human infection with Plasmodium knowlesi has been recently discovered in Thailand. To investigate the prevalence of this malaria species, a molecular-based survey was performed. Methods. Blood samples from 1874 patients were tested for Plasmodium species by microscopy and nested polymerase chain reaction. P. knowlesi was characterized by sequencing the merozoite surface protein 1 gene (msp-1). Results. Of all Plasmodium species identified, P. falciparum, P. vivax, P. malariae, P. ovale, and P. knowlesi contributed 43.52%, 68.08%, 1.37%, 1.03%, and 0.57%, respectively. Mixed-species infections were more common in northwestern and southwestern regions bordering Myanmar (23%-24%) than in eastern and southern areas (3%- 5%). In northwestern and southwestern regions, mixed-species infections had a significantly higher prevalence in dry than in rainy seasons (P < .001). P. knowlesi was found in 10 patients, mostly from southern and southwestern areas-9 were coinfected with either P. falciparum or P. vivax. Most of the P. knowlesi Thai isolates were more closely related to isolates from macaques than to isolates from Sarawak patients. The msp-1 sequences of isolates from the same area of endemicity differed and possessed novel sequences, indicating genetic polymorphism in P. knowlesi infecting humans. Conclusions. This survey highlights the widespread distribution of P. knowlesi in Thailand, albeit at low prevalence and mostly occurring as cryptic infections. © 2009 by the Infectious Diseases Society of America. 2018-09-13T07:02:27Z 2018-09-13T07:02:27Z 2009-04-15 Article Journal of Infectious Diseases. Vol.199, No.8 (2009), 1143-1150 10.1086/597414 00221899 2-s2.0-65649123388 https://repository.li.mahidol.ac.th/handle/123456789/28115 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=65649123388&origin=inward |
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Medicine Chaturong Putaporntip Thongchai Hongsrimuang Sunee Seethamchai Teerayot Kobasa Kriengsak Limkittikul Liwang Cui Somchai Jongwutiwes Differential prevalence of plasmodium infections and cryptic plasmodium knowlesi malaria in humans in Thailand |
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Background. A case of human infection with Plasmodium knowlesi has been recently discovered in Thailand. To investigate the prevalence of this malaria species, a molecular-based survey was performed. Methods. Blood samples from 1874 patients were tested for Plasmodium species by microscopy and nested polymerase chain reaction. P. knowlesi was characterized by sequencing the merozoite surface protein 1 gene (msp-1). Results. Of all Plasmodium species identified, P. falciparum, P. vivax, P. malariae, P. ovale, and P. knowlesi contributed 43.52%, 68.08%, 1.37%, 1.03%, and 0.57%, respectively. Mixed-species infections were more common in northwestern and southwestern regions bordering Myanmar (23%-24%) than in eastern and southern areas (3%- 5%). In northwestern and southwestern regions, mixed-species infections had a significantly higher prevalence in dry than in rainy seasons (P < .001). P. knowlesi was found in 10 patients, mostly from southern and southwestern areas-9 were coinfected with either P. falciparum or P. vivax. Most of the P. knowlesi Thai isolates were more closely related to isolates from macaques than to isolates from Sarawak patients. The msp-1 sequences of isolates from the same area of endemicity differed and possessed novel sequences, indicating genetic polymorphism in P. knowlesi infecting humans. Conclusions. This survey highlights the widespread distribution of P. knowlesi in Thailand, albeit at low prevalence and mostly occurring as cryptic infections. © 2009 by the Infectious Diseases Society of America. |
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Molecular Biology of Malaria and Opportunistic Parasites Research Unit |
author_facet |
Molecular Biology of Malaria and Opportunistic Parasites Research Unit Chaturong Putaporntip Thongchai Hongsrimuang Sunee Seethamchai Teerayot Kobasa Kriengsak Limkittikul Liwang Cui Somchai Jongwutiwes |
format |
Article |
author |
Chaturong Putaporntip Thongchai Hongsrimuang Sunee Seethamchai Teerayot Kobasa Kriengsak Limkittikul Liwang Cui Somchai Jongwutiwes |
author_sort |
Chaturong Putaporntip |
title |
Differential prevalence of plasmodium infections and cryptic plasmodium knowlesi malaria in humans in Thailand |
title_short |
Differential prevalence of plasmodium infections and cryptic plasmodium knowlesi malaria in humans in Thailand |
title_full |
Differential prevalence of plasmodium infections and cryptic plasmodium knowlesi malaria in humans in Thailand |
title_fullStr |
Differential prevalence of plasmodium infections and cryptic plasmodium knowlesi malaria in humans in Thailand |
title_full_unstemmed |
Differential prevalence of plasmodium infections and cryptic plasmodium knowlesi malaria in humans in Thailand |
title_sort |
differential prevalence of plasmodium infections and cryptic plasmodium knowlesi malaria in humans in thailand |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/28115 |
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1763487233365508096 |