Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine need...

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Main Authors: Kampon Kaeoket, Panida Chanapiwat, Padet Tummaruk, Mongkol Techakumphu
Other Authors: Mahidol University
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/29543
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spelling th-mahidol.295432018-09-24T16:21:41Z Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen Kampon Kaeoket Panida Chanapiwat Padet Tummaruk Mongkol Techakumphu Mahidol University Faculty of Veterinary Science Medicine Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with various concentrations of L-cysteine to reach 0 mmol L 1 (group I, control), 5 mmol L 1 (group II), 10 mmol L 1 (group III) and 15 mmol L 1 (group IV). Semen suspensions were loaded in straws (0.5 mL) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability using SYBR-14/EthD-1 staining and acrosome integrity using FITC-PNA/EthD-1 staining. There was a significantly higher (P<0.01) percentage of progressive motility, viability and acrosomal integrity in two L-cysteine-supplemented groups (group II and group III) compared with the control. There was a biphasic effect of L-cysteine, with the highest percentage of progressive motility, viability and acrosomal integrity in group III. In conclusion, 5 or 10 mmol L 1 was the optimum concentration of L-cysteine to be added to the LEY extender for improving the quality of frozen-thawed boar semen. © 2010 AJA, SIMM & SJTU All rights reserved. 2018-09-24T09:21:41Z 2018-09-24T09:21:41Z 2010-09-01 Article Asian Journal of Andrology. Vol.12, No.5 (2010), 760-765 10.1038/aja.2010.48 17457262 1008682X 2-s2.0-77956425993 https://repository.li.mahidol.ac.th/handle/123456789/29543 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77956425993&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Medicine
spellingShingle Medicine
Kampon Kaeoket
Panida Chanapiwat
Padet Tummaruk
Mongkol Techakumphu
Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen
description Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with various concentrations of L-cysteine to reach 0 mmol L 1 (group I, control), 5 mmol L 1 (group II), 10 mmol L 1 (group III) and 15 mmol L 1 (group IV). Semen suspensions were loaded in straws (0.5 mL) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability using SYBR-14/EthD-1 staining and acrosome integrity using FITC-PNA/EthD-1 staining. There was a significantly higher (P<0.01) percentage of progressive motility, viability and acrosomal integrity in two L-cysteine-supplemented groups (group II and group III) compared with the control. There was a biphasic effect of L-cysteine, with the highest percentage of progressive motility, viability and acrosomal integrity in group III. In conclusion, 5 or 10 mmol L 1 was the optimum concentration of L-cysteine to be added to the LEY extender for improving the quality of frozen-thawed boar semen. © 2010 AJA, SIMM & SJTU All rights reserved.
author2 Mahidol University
author_facet Mahidol University
Kampon Kaeoket
Panida Chanapiwat
Padet Tummaruk
Mongkol Techakumphu
format Article
author Kampon Kaeoket
Panida Chanapiwat
Padet Tummaruk
Mongkol Techakumphu
author_sort Kampon Kaeoket
title Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen
title_short Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen
title_full Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen
title_fullStr Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen
title_full_unstemmed Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen
title_sort supplemental effect of varying l-cysteine concentrations on the quality of cryopreserved boar semen
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/29543
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