Improvement of culture conditions for long‑term in vitro culture of Plasmodium vivax
Background: The study of the biology, transmission and pathogenesis of Plasmodium vivax is hindered due to the lack of a robustly propagating, continuous culture of this parasite. The current culture system for P. vivax parasites still suffered from consistency and difficulties in long-term mainte...
Saved in:
Main Authors: | , , , , , , , |
---|---|
Other Authors: | |
Format: | Other |
Language: | English |
Published: |
2017
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/3081 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
Language: | English |
id |
th-mahidol.3081 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.30812023-03-31T06:59:44Z Improvement of culture conditions for long‑term in vitro culture of Plasmodium vivax Wanlapa Roobsoong Tharinjaroen, Chayada S Nattawan Rachaphaew Porpimon Chobson Louis Schofield Liwang Cui Adams, John H Jetsumon Sattabongkot Mahidol University. Faculty of Tropical Medicine. Mahidol Vivax Research Unit Open Access article Plasmodium vivax In vitro culture Invasion assay Membrane feeding assay Culture medium Reticulocytes Background: The study of the biology, transmission and pathogenesis of Plasmodium vivax is hindered due to the lack of a robustly propagating, continuous culture of this parasite. The current culture system for P. vivax parasites still suffered from consistency and difficulties in long-term maintenance of parasites in culture and for providing sufficient biological materials for studying parasite biology. Therefore, further improvement of culture conditions for P. vivax is needed. Methods: Clinical samples were collected from patients diagnosed with P. vivax in western Thailand. Leukocytedepleted P. vivax infected blood samples were cultured in a modified McCoy’s 5A medium at 5% haematocrit under hypoxic condition (5% O2, 5% CO2, and 90% N2). Reticulocytes purified from adult peripheral blood were added daily to maintain 4% reticulocytes. Parasites were detected by microscopic examination of Giemsa-stained smears and molecular methods. Results: The effects of culture variables were first analysed in order to improve the culture conditions for P. vivax. Through analysis of the sources of host reticulocytes and nutrients of culture medium, the culture conditions better supporting in vitro growth and maturation of the parasites were identified. Using this system, three of 30 isolates could be maintained in vitro for over 26 months albeit parasite density is low. Conclusions: Based on the analysis of different culture variables, an improved and feasible protocol for continuous culture of P. vivax was developed. 2017-11-08T03:31:19Z 2017-11-08T03:31:19Z 2017-11-08 2015 Other Malaria Journal. Vol.14, (2015), 297 10.1186/s12936-015-0815-z https://repository.li.mahidol.ac.th/handle/123456789/3081 eng Mahidol University BioMed Central application/pdf |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
language |
English |
topic |
Open Access article Plasmodium vivax In vitro culture Invasion assay Membrane feeding assay Culture medium Reticulocytes |
spellingShingle |
Open Access article Plasmodium vivax In vitro culture Invasion assay Membrane feeding assay Culture medium Reticulocytes Wanlapa Roobsoong Tharinjaroen, Chayada S Nattawan Rachaphaew Porpimon Chobson Louis Schofield Liwang Cui Adams, John H Jetsumon Sattabongkot Improvement of culture conditions for long‑term in vitro culture of Plasmodium vivax |
description |
Background: The study of the biology, transmission and pathogenesis of Plasmodium vivax is hindered due to the
lack of a robustly propagating, continuous culture of this parasite. The current culture system for P. vivax parasites still
suffered from consistency and difficulties in long-term maintenance of parasites in culture and for providing sufficient
biological materials for studying parasite biology. Therefore, further improvement of culture conditions for P. vivax is
needed.
Methods: Clinical samples were collected from patients diagnosed with P. vivax in western Thailand. Leukocytedepleted
P. vivax infected blood samples were cultured in a modified McCoy’s 5A medium at 5% haematocrit under
hypoxic condition (5% O2, 5% CO2, and 90% N2). Reticulocytes purified from adult peripheral blood were added daily
to maintain 4% reticulocytes. Parasites were detected by microscopic examination of Giemsa-stained smears and
molecular methods.
Results: The effects of culture variables were first analysed in order to improve the culture conditions for P. vivax.
Through analysis of the sources of host reticulocytes and nutrients of culture medium, the culture conditions better
supporting in vitro growth and maturation of the parasites were identified. Using this system, three of 30 isolates
could be maintained in vitro for over 26 months albeit parasite density is low.
Conclusions: Based on the analysis of different culture variables, an improved and feasible protocol for continuous
culture of P. vivax was developed. |
author2 |
Mahidol University. Faculty of Tropical Medicine. Mahidol Vivax Research Unit |
author_facet |
Mahidol University. Faculty of Tropical Medicine. Mahidol Vivax Research Unit Wanlapa Roobsoong Tharinjaroen, Chayada S Nattawan Rachaphaew Porpimon Chobson Louis Schofield Liwang Cui Adams, John H Jetsumon Sattabongkot |
format |
Other |
author |
Wanlapa Roobsoong Tharinjaroen, Chayada S Nattawan Rachaphaew Porpimon Chobson Louis Schofield Liwang Cui Adams, John H Jetsumon Sattabongkot |
author_sort |
Wanlapa Roobsoong |
title |
Improvement of culture conditions for long‑term in vitro culture of Plasmodium vivax |
title_short |
Improvement of culture conditions for long‑term in vitro culture of Plasmodium vivax |
title_full |
Improvement of culture conditions for long‑term in vitro culture of Plasmodium vivax |
title_fullStr |
Improvement of culture conditions for long‑term in vitro culture of Plasmodium vivax |
title_full_unstemmed |
Improvement of culture conditions for long‑term in vitro culture of Plasmodium vivax |
title_sort |
improvement of culture conditions for long‑term in vitro culture of plasmodium vivax |
publishDate |
2017 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/3081 |
_version_ |
1763497621509373952 |