Heat stabilization of blood spot samples for determination of metabolically unstable drug compounds
Background: Sample stability is critical for accurate analysis of drug compounds in biosamples. The use of additives to eradicate the enzymatic activity causing loss of these analytes has its limitations. Results: A novel technique for sample stabilization by rapid, high-temperature heating was used...
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th-mahidol.314262018-10-19T12:46:10Z Heat stabilization of blood spot samples for determination of metabolically unstable drug compounds Daniel Blessborn Karl Sköld David Zeeberg Karnrawee Kaewkhao Olof Sköld Martin Ahnoff Mahidol University Nuffield Department of Clinical Medicine Denator AB AstraZeneca Sweden Goteborgs Universitet Biochemistry, Genetics and Molecular Biology Chemistry Health Professions Pharmacology, Toxicology and Pharmaceutics Background: Sample stability is critical for accurate analysis of drug compounds in biosamples. The use of additives to eradicate the enzymatic activity causing loss of these analytes has its limitations. Results: A novel technique for sample stabilization by rapid, high-temperature heating was used. The stability of six commercial drugs in blood and blood spots was investigated under various conditions with or without heat stabilization at 95°C. Oseltamivir, cefotaxime and ribavirin were successfully stabilized by heating whereas significant losses were seen in unheated samples. Amodiaquine was stable with and without heating. Artemether and dihydroartemisinin were found to be very heat sensitive and began to decompose even at 60°C. Conclusion: Heat stabilization is a viable technique to maintain analytes in blood spot samples, without the use of chemical additives, by stopping the enzymatic activity that causes sample degradation. © 2013 Denator AB. 2018-10-19T04:44:08Z 2018-10-19T04:44:08Z 2013-01-01 Article Bioanalysis. Vol.5, No.1 (2013), 31-39 10.4155/bio.12.294 17576199 17576180 2-s2.0-84871586789 https://repository.li.mahidol.ac.th/handle/123456789/31426 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84871586789&origin=inward |
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Biochemistry, Genetics and Molecular Biology Chemistry Health Professions Pharmacology, Toxicology and Pharmaceutics Daniel Blessborn Karl Sköld David Zeeberg Karnrawee Kaewkhao Olof Sköld Martin Ahnoff Heat stabilization of blood spot samples for determination of metabolically unstable drug compounds |
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Background: Sample stability is critical for accurate analysis of drug compounds in biosamples. The use of additives to eradicate the enzymatic activity causing loss of these analytes has its limitations. Results: A novel technique for sample stabilization by rapid, high-temperature heating was used. The stability of six commercial drugs in blood and blood spots was investigated under various conditions with or without heat stabilization at 95°C. Oseltamivir, cefotaxime and ribavirin were successfully stabilized by heating whereas significant losses were seen in unheated samples. Amodiaquine was stable with and without heating. Artemether and dihydroartemisinin were found to be very heat sensitive and began to decompose even at 60°C. Conclusion: Heat stabilization is a viable technique to maintain analytes in blood spot samples, without the use of chemical additives, by stopping the enzymatic activity that causes sample degradation. © 2013 Denator AB. |
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Mahidol University |
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Mahidol University Daniel Blessborn Karl Sköld David Zeeberg Karnrawee Kaewkhao Olof Sköld Martin Ahnoff |
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Article |
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Daniel Blessborn Karl Sköld David Zeeberg Karnrawee Kaewkhao Olof Sköld Martin Ahnoff |
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Daniel Blessborn |
title |
Heat stabilization of blood spot samples for determination of metabolically unstable drug compounds |
title_short |
Heat stabilization of blood spot samples for determination of metabolically unstable drug compounds |
title_full |
Heat stabilization of blood spot samples for determination of metabolically unstable drug compounds |
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Heat stabilization of blood spot samples for determination of metabolically unstable drug compounds |
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Heat stabilization of blood spot samples for determination of metabolically unstable drug compounds |
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heat stabilization of blood spot samples for determination of metabolically unstable drug compounds |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/31426 |
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