Melanization reaction products of shrimp display antimicrobial properties against their major bacterial and fungal pathogens
Melanization is a rapid defense mechanism in invertebrates. The substrate specificity of phenoloxidases (POs) and the role of melanization reaction products were investigated in the black tiger shrimp, Penaeus monodon. Two PmPOs (PmproPO1 and PmproPO2) were found to display a substrate specificity t...
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th-mahidol.334482018-11-09T09:25:54Z Melanization reaction products of shrimp display antimicrobial properties against their major bacterial and fungal pathogens Walaiporn Charoensapsri Piti Amparyup Chawapat Suriyachan Anchalee Tassanakajon Chulalongkorn University Mahidol University Thailand National Center for Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Melanization is a rapid defense mechanism in invertebrates. The substrate specificity of phenoloxidases (POs) and the role of melanization reaction products were investigated in the black tiger shrimp, Penaeus monodon. Two PmPOs (PmproPO1 and PmproPO2) were found to display a substrate specificity towards monophenols and diphenols, and exhibit relatively weak activity against 5,6-dihydroxyindole (DHI). Systemic infection of the PmproPO1/2 co-silenced shrimp with the fungus, Fusarium solani, led to a significantly increased mortality, suggesting an important role of PmproPOs in shrimp's defense against fungal infection. Using l-DOPA, dopamine or DHI as a substrate, the melanization reaction products exhibited in vitro antimicrobial activities towards Gram-negative bacteria (Vibrio harveyi and Vibrio parahaemolyticus) and Gram-positive bacteria (Bacillus subtilis), whereas the lower effect was detected against the fungus (F. solani). SEM analysis revealed the morphological changes and damage of cell membranes of V. harveyi and F. solani after treatment with shrimp melanization reaction products. Together, these findings demonstrate the crucial functions of the proPO system and the importance of melanization reaction products in the shrimp's immune defense. © 2014 Elsevier Ltd. 2018-11-09T01:59:05Z 2018-11-09T01:59:05Z 2014-01-01 Article Developmental and Comparative Immunology. Vol.47, No.1 (2014), 150-159 10.1016/j.dci.2014.07.010 18790089 0145305X 2-s2.0-84905660372 https://repository.li.mahidol.ac.th/handle/123456789/33448 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84905660372&origin=inward |
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Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Walaiporn Charoensapsri Piti Amparyup Chawapat Suriyachan Anchalee Tassanakajon Melanization reaction products of shrimp display antimicrobial properties against their major bacterial and fungal pathogens |
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Melanization is a rapid defense mechanism in invertebrates. The substrate specificity of phenoloxidases (POs) and the role of melanization reaction products were investigated in the black tiger shrimp, Penaeus monodon. Two PmPOs (PmproPO1 and PmproPO2) were found to display a substrate specificity towards monophenols and diphenols, and exhibit relatively weak activity against 5,6-dihydroxyindole (DHI). Systemic infection of the PmproPO1/2 co-silenced shrimp with the fungus, Fusarium solani, led to a significantly increased mortality, suggesting an important role of PmproPOs in shrimp's defense against fungal infection. Using l-DOPA, dopamine or DHI as a substrate, the melanization reaction products exhibited in vitro antimicrobial activities towards Gram-negative bacteria (Vibrio harveyi and Vibrio parahaemolyticus) and Gram-positive bacteria (Bacillus subtilis), whereas the lower effect was detected against the fungus (F. solani). SEM analysis revealed the morphological changes and damage of cell membranes of V. harveyi and F. solani after treatment with shrimp melanization reaction products. Together, these findings demonstrate the crucial functions of the proPO system and the importance of melanization reaction products in the shrimp's immune defense. © 2014 Elsevier Ltd. |
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Chulalongkorn University |
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Chulalongkorn University Walaiporn Charoensapsri Piti Amparyup Chawapat Suriyachan Anchalee Tassanakajon |
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Article |
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Walaiporn Charoensapsri Piti Amparyup Chawapat Suriyachan Anchalee Tassanakajon |
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Walaiporn Charoensapsri |
title |
Melanization reaction products of shrimp display antimicrobial properties against their major bacterial and fungal pathogens |
title_short |
Melanization reaction products of shrimp display antimicrobial properties against their major bacterial and fungal pathogens |
title_full |
Melanization reaction products of shrimp display antimicrobial properties against their major bacterial and fungal pathogens |
title_fullStr |
Melanization reaction products of shrimp display antimicrobial properties against their major bacterial and fungal pathogens |
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Melanization reaction products of shrimp display antimicrobial properties against their major bacterial and fungal pathogens |
title_sort |
melanization reaction products of shrimp display antimicrobial properties against their major bacterial and fungal pathogens |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/33448 |
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1763494779197325312 |