Development of three-dimensional (3D) full automated paper-based elisa devices for easy production and preservation stability

Development of three-dimensional (3D) full automated paper-based elisa devices for easy production and preservation stability

© 15CBMS-0001. A new 3D design of paper-based device on nitrocellulose (NC) membrane with the BCIP/NBT substrate kept on soluble solid for fully automatic multistep sequencing of sandwich ELISA has been developed to improve sensitivity, mass productivity and lifetime of the device. The detection lim...

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Bibliographic Details
Main Authors: Thanyaporn Kiatkumjorn, Kingkarn Pungjunun, Amara Apilux, Phan Trong Tue, Orawon Chailapakul, Yuzuru Takamura
Other Authors: Japan Advanced Institute of Science and Technology
Format: Conference or Workshop Item
Published: 2018
Subjects:
Engineering
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/35968
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Institution: Mahidol University
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Summary:© 15CBMS-0001. A new 3D design of paper-based device on nitrocellulose (NC) membrane with the BCIP/NBT substrate kept on soluble solid for fully automatic multistep sequencing of sandwich ELISA has been developed to improve sensitivity, mass productivity and lifetime of the device. The detection limit of the 3D device with the BCIP/NBT substrate kept on soluble solid was found to be 1.01 mIU/mL which is lower than that with direct immobilization (10.7 mIU/mL). This device can be applied for a greater variety of clinical (point of care assays) or environmental analysis.

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