Microparticle and anti-influenza activity in human respiratory secretion
© 2017 Suptawiwat et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Respiratory secretions, such as saliva...
Saved in:
Main Authors: | , , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2018
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/41408 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.41408 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.414082019-03-14T15:02:22Z Microparticle and anti-influenza activity in human respiratory secretion Ornpreya Suptawiwat Kanyarat Ruangrung Chompunuch Boonarkart Pilaipan Puthavathana Kittipong Maneechotesuwan Komgrid Charngkaew Nusara Chomanee Prasert Auewarakul Mahidol University Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology © 2017 Suptawiwat et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Respiratory secretions, such as saliva and bronchoalveolar fluid, contain anti-influenza activity. Multiple soluble factors have been described that exert anti-influenza activity and are believed to be responsible for the anti-influenza activity in respiratory secretions. It was previously shown that a bronchial epithelial cell culture could produce exosome-like particles with anti-influenza activity. Whether such extracellular vesicles in respiratory secretions have anti-influenza activity is unknown. Therefore, we characterized bronchoalveolar lavage fluid and found microparticles, which mostly stained positive for epithelial cell markers and both a2,3- and a2,6-linked sialic acid. Microparticles were purified from bronchoalveolar lavage fluid and shown to exhibit anti-influenza activity by a hemagglutination inhibition (HI) assay and a neutralization (NT) assay. In addition, physical binding between influenza virions and microparticles was demonstrated by electron microscopy. These findings indicate that respiratory microparticles containing viral receptors can exert anti-viral activity by probably trapping viral particles. This innate mechanism may play an important role in the defense against respiratory viruses. 2018-12-21T06:26:39Z 2019-03-14T08:02:22Z 2018-12-21T06:26:39Z 2019-03-14T08:02:22Z 2017-08-01 Article PLoS ONE. Vol.12, No.8 (2017) 10.1371/journal.pone.0183717 19326203 2-s2.0-85028468834 https://repository.li.mahidol.ac.th/handle/123456789/41408 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85028468834&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology |
spellingShingle |
Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Ornpreya Suptawiwat Kanyarat Ruangrung Chompunuch Boonarkart Pilaipan Puthavathana Kittipong Maneechotesuwan Komgrid Charngkaew Nusara Chomanee Prasert Auewarakul Microparticle and anti-influenza activity in human respiratory secretion |
description |
© 2017 Suptawiwat et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Respiratory secretions, such as saliva and bronchoalveolar fluid, contain anti-influenza activity. Multiple soluble factors have been described that exert anti-influenza activity and are believed to be responsible for the anti-influenza activity in respiratory secretions. It was previously shown that a bronchial epithelial cell culture could produce exosome-like particles with anti-influenza activity. Whether such extracellular vesicles in respiratory secretions have anti-influenza activity is unknown. Therefore, we characterized bronchoalveolar lavage fluid and found microparticles, which mostly stained positive for epithelial cell markers and both a2,3- and a2,6-linked sialic acid. Microparticles were purified from bronchoalveolar lavage fluid and shown to exhibit anti-influenza activity by a hemagglutination inhibition (HI) assay and a neutralization (NT) assay. In addition, physical binding between influenza virions and microparticles was demonstrated by electron microscopy. These findings indicate that respiratory microparticles containing viral receptors can exert anti-viral activity by probably trapping viral particles. This innate mechanism may play an important role in the defense against respiratory viruses. |
author2 |
Mahidol University |
author_facet |
Mahidol University Ornpreya Suptawiwat Kanyarat Ruangrung Chompunuch Boonarkart Pilaipan Puthavathana Kittipong Maneechotesuwan Komgrid Charngkaew Nusara Chomanee Prasert Auewarakul |
format |
Article |
author |
Ornpreya Suptawiwat Kanyarat Ruangrung Chompunuch Boonarkart Pilaipan Puthavathana Kittipong Maneechotesuwan Komgrid Charngkaew Nusara Chomanee Prasert Auewarakul |
author_sort |
Ornpreya Suptawiwat |
title |
Microparticle and anti-influenza activity in human respiratory secretion |
title_short |
Microparticle and anti-influenza activity in human respiratory secretion |
title_full |
Microparticle and anti-influenza activity in human respiratory secretion |
title_fullStr |
Microparticle and anti-influenza activity in human respiratory secretion |
title_full_unstemmed |
Microparticle and anti-influenza activity in human respiratory secretion |
title_sort |
microparticle and anti-influenza activity in human respiratory secretion |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/41408 |
_version_ |
1763496361880190976 |