High-performance liquid chromatography with time-programmed fluorescence detection for the quantification of Levofloxacin in human plasma and cerebrospinal fluid in adults with tuberculous meningitis

© 2017 The Authors An accurate and reliable high-performance liquid chromatography with time-programmed fluorescence detection was developed and validated to measure levofloxacin in human plasma and cerebrospinal fluid (CSF). After solid phase extraction process using Evolute®ABN 96 fixed well plate...

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Main Authors: Pham Van Toi, Thomas Pouplin, Nguyen Duc Khanh Tho, Pham Nguyen Phuong, Tran Thi Hong Chau, Nguyen Thuy Thuong Thuong, Dorothee Heemskerk, Tran Tinh Hien, Guy E. Thwaites
Other Authors: UCL
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Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/41736
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spelling th-mahidol.417362019-03-14T15:02:43Z High-performance liquid chromatography with time-programmed fluorescence detection for the quantification of Levofloxacin in human plasma and cerebrospinal fluid in adults with tuberculous meningitis Pham Van Toi Thomas Pouplin Nguyen Duc Khanh Tho Pham Nguyen Phuong Tran Thi Hong Chau Nguyen Thuy Thuong Thuong Dorothee Heemskerk Tran Tinh Hien Guy E. Thwaites UCL Nuffield Department of Clinical Medicine Mahidol University Biochemistry, Genetics and Molecular Biology Chemistry © 2017 The Authors An accurate and reliable high-performance liquid chromatography with time-programmed fluorescence detection was developed and validated to measure levofloxacin in human plasma and cerebrospinal fluid (CSF). After solid phase extraction process using Evolute®ABN 96 fixed well plate; levofloxacin and internal standard-enoxacin were separated using a mobile phase consisting of phosphate buffer 10 mM with 0.025% triethylamine pH 3.0 - acetonitrile (88:12, v/v) on a Purosphere RP-8e column (5 μm, 125 × 4.0mm) at a flow rate of 1.2 mL/min at 35 °C. The excitation/emission wavelengths were set to 269/400 nm and 294/500 nm, for enoxacin and levofloxacin, respectively. The method was linear over the concentration range of 0.02 to 20.0 μg/mL with a limit of detection of 0.01 μg/mL. The relative standard deviation of intra-assay and inter-assay precision for levofloxacin at four quality controls concentrations (0.02, 0.06, 3.0 and 15.0 μg/mL) were less than 7% and the accuracies ranged from 96.75% to 101.9% in plasma, and from 93.00% to 98.67% in CSF. The validated method was successfully applied to quantify levofloxacin in a considerable quantity of plasma (826) and CSF (477) samples collected from 232 tuberculous meningitis patients, and the preliminary intensive pharmacokinetics analysis from 14 tuberculous meningitis patients in Vietnam is described in this paper. 2018-12-21T06:43:32Z 2019-03-14T08:02:43Z 2018-12-21T06:43:32Z 2019-03-14T08:02:43Z 2017-09-01 Article Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. Vol.1061-1062, (2017), 256-262 10.1016/j.jchromb.2017.07.032 1873376X 15700232 2-s2.0-85025606536 https://repository.li.mahidol.ac.th/handle/123456789/41736 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85025606536&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Pham Van Toi
Thomas Pouplin
Nguyen Duc Khanh Tho
Pham Nguyen Phuong
Tran Thi Hong Chau
Nguyen Thuy Thuong Thuong
Dorothee Heemskerk
Tran Tinh Hien
Guy E. Thwaites
High-performance liquid chromatography with time-programmed fluorescence detection for the quantification of Levofloxacin in human plasma and cerebrospinal fluid in adults with tuberculous meningitis
description © 2017 The Authors An accurate and reliable high-performance liquid chromatography with time-programmed fluorescence detection was developed and validated to measure levofloxacin in human plasma and cerebrospinal fluid (CSF). After solid phase extraction process using Evolute®ABN 96 fixed well plate; levofloxacin and internal standard-enoxacin were separated using a mobile phase consisting of phosphate buffer 10 mM with 0.025% triethylamine pH 3.0 - acetonitrile (88:12, v/v) on a Purosphere RP-8e column (5 μm, 125 × 4.0mm) at a flow rate of 1.2 mL/min at 35 °C. The excitation/emission wavelengths were set to 269/400 nm and 294/500 nm, for enoxacin and levofloxacin, respectively. The method was linear over the concentration range of 0.02 to 20.0 μg/mL with a limit of detection of 0.01 μg/mL. The relative standard deviation of intra-assay and inter-assay precision for levofloxacin at four quality controls concentrations (0.02, 0.06, 3.0 and 15.0 μg/mL) were less than 7% and the accuracies ranged from 96.75% to 101.9% in plasma, and from 93.00% to 98.67% in CSF. The validated method was successfully applied to quantify levofloxacin in a considerable quantity of plasma (826) and CSF (477) samples collected from 232 tuberculous meningitis patients, and the preliminary intensive pharmacokinetics analysis from 14 tuberculous meningitis patients in Vietnam is described in this paper.
author2 UCL
author_facet UCL
Pham Van Toi
Thomas Pouplin
Nguyen Duc Khanh Tho
Pham Nguyen Phuong
Tran Thi Hong Chau
Nguyen Thuy Thuong Thuong
Dorothee Heemskerk
Tran Tinh Hien
Guy E. Thwaites
format Article
author Pham Van Toi
Thomas Pouplin
Nguyen Duc Khanh Tho
Pham Nguyen Phuong
Tran Thi Hong Chau
Nguyen Thuy Thuong Thuong
Dorothee Heemskerk
Tran Tinh Hien
Guy E. Thwaites
author_sort Pham Van Toi
title High-performance liquid chromatography with time-programmed fluorescence detection for the quantification of Levofloxacin in human plasma and cerebrospinal fluid in adults with tuberculous meningitis
title_short High-performance liquid chromatography with time-programmed fluorescence detection for the quantification of Levofloxacin in human plasma and cerebrospinal fluid in adults with tuberculous meningitis
title_full High-performance liquid chromatography with time-programmed fluorescence detection for the quantification of Levofloxacin in human plasma and cerebrospinal fluid in adults with tuberculous meningitis
title_fullStr High-performance liquid chromatography with time-programmed fluorescence detection for the quantification of Levofloxacin in human plasma and cerebrospinal fluid in adults with tuberculous meningitis
title_full_unstemmed High-performance liquid chromatography with time-programmed fluorescence detection for the quantification of Levofloxacin in human plasma and cerebrospinal fluid in adults with tuberculous meningitis
title_sort high-performance liquid chromatography with time-programmed fluorescence detection for the quantification of levofloxacin in human plasma and cerebrospinal fluid in adults with tuberculous meningitis
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/41736
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