Selective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 protein
© 2017 Elsevier Inc. Influenza A viruses contain eight single-stranded, negative-sense RNA segments as viral genomes in the form of viral ribonucleoproteins (vRNPs). During genome replication in the nucleus, positive-sense complementary RNPs (cRNPs) are produced as replicative intermediates, which a...
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th-mahidol.428222019-03-14T15:03:51Z Selective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 protein Chutikarn Chaimayo Tsuyoshi Hayashi Andrew Underwood Erin Hodges Toru Takimoto University of Rochester Medical Center Mahidol University National Center for Immunization and Respiratory Diseases Immunology and Microbiology © 2017 Elsevier Inc. Influenza A viruses contain eight single-stranded, negative-sense RNA segments as viral genomes in the form of viral ribonucleoproteins (vRNPs). During genome replication in the nucleus, positive-sense complementary RNPs (cRNPs) are produced as replicative intermediates, which are not incorporated into progeny virions. To analyze the mechanism of selective vRNP incorporation into progeny virions, we quantified vRNPs and cRNPs in the nuclear and cytosolic fractions of infected cells, using a strand-specific qRT-PCR. Unexpectedly, we found that cRNPs were also exported to the cytoplasm. This export was chromosome region maintenance 1 (CRM1)-independent unlike that of vRNPs. Although both vRNPs and cRNPs were present in the cytosol, viral matrix (M1) protein, a key regulator for viral assembly, preferentially bound vRNPs over cRNPs. These results indicate that influenza A viruses selectively uptake cytosolic vRNPs through a specific interaction with M1 during viral assembly. 2018-12-21T07:58:55Z 2019-03-14T08:03:51Z 2018-12-21T07:58:55Z 2019-03-14T08:03:51Z 2017-05-01 Article Virology. Vol.505, (2017), 23-32 10.1016/j.virol.2017.02.008 10960341 00426822 2-s2.0-85013054834 https://repository.li.mahidol.ac.th/handle/123456789/42822 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85013054834&origin=inward |
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Immunology and Microbiology Chutikarn Chaimayo Tsuyoshi Hayashi Andrew Underwood Erin Hodges Toru Takimoto Selective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 protein |
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© 2017 Elsevier Inc. Influenza A viruses contain eight single-stranded, negative-sense RNA segments as viral genomes in the form of viral ribonucleoproteins (vRNPs). During genome replication in the nucleus, positive-sense complementary RNPs (cRNPs) are produced as replicative intermediates, which are not incorporated into progeny virions. To analyze the mechanism of selective vRNP incorporation into progeny virions, we quantified vRNPs and cRNPs in the nuclear and cytosolic fractions of infected cells, using a strand-specific qRT-PCR. Unexpectedly, we found that cRNPs were also exported to the cytoplasm. This export was chromosome region maintenance 1 (CRM1)-independent unlike that of vRNPs. Although both vRNPs and cRNPs were present in the cytosol, viral matrix (M1) protein, a key regulator for viral assembly, preferentially bound vRNPs over cRNPs. These results indicate that influenza A viruses selectively uptake cytosolic vRNPs through a specific interaction with M1 during viral assembly. |
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University of Rochester Medical Center |
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University of Rochester Medical Center Chutikarn Chaimayo Tsuyoshi Hayashi Andrew Underwood Erin Hodges Toru Takimoto |
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Article |
author |
Chutikarn Chaimayo Tsuyoshi Hayashi Andrew Underwood Erin Hodges Toru Takimoto |
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Chutikarn Chaimayo |
title |
Selective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 protein |
title_short |
Selective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 protein |
title_full |
Selective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 protein |
title_fullStr |
Selective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 protein |
title_full_unstemmed |
Selective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 protein |
title_sort |
selective incorporation of vrnp into influenza a virions determined by its specific interaction with m1 protein |
publishDate |
2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/42822 |
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1763492067865001984 |