Encapsulation of the reductase component of p-hydroxyphenylacetate hydroxylase in poly(lactide-co-glycolide) nanoparticles by three different emulsification techniques

Encapsulation of the reductase component of p-hydroxyphenylacetate hydroxylase in poly(lactide-co-glycolide) nanoparticles by three different emulsification techniques

© The Institution of Engineering and Technology 2017. p-Hydroxyphenylacetate 3-hydroxylase component 1 (C 1 ) is a useful enzyme for generating reduced flavin and NAD + intermediates. In this study, poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) were used to encapsulate the C 1 (PLGA-C 1 NPs...

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Bibliographic Details
Main Authors: Komkrich Sawasdee, Jeerus Sucharitakul, Taweesak Dhammaraj, Nuttawee Niamsiri, Pimchai Chaiyen, Kanlaya Prapainop
Other Authors: Vidyasirimedhi Institute of Science and Technology
Format: Article
Published: 2019
Subjects:
Biochemistry, Genetics and Molecular Biology
Engineering
Materials Science
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/45153
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Institution: Mahidol University
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Summary:© The Institution of Engineering and Technology 2017. p-Hydroxyphenylacetate 3-hydroxylase component 1 (C 1 ) is a useful enzyme for generating reduced flavin and NAD + intermediates. In this study, poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) were used to encapsulate the C 1 (PLGA-C 1 NPs). Enzymatic activity, stability, and reusability of PLGA-C 1 NPs prepared using three different methods [oil in water (o/w), water in oil in water (w/o/w), and solid in oil in water (s/o/w)] were compared. The s/o/w provided the optimal conditions for encapsulation of C 1 (PLGA-C 1,s NPs), giving the highest enzyme activity, stability, and reusability. The s/o/w method improves enzyme activity ∼11 and 9-fold compared to w/o/w (PLGA-C 1,w NPs) and o/w (PLGA-C 1,o NPs). In addition, s/o/w prepared PLGA-C 1,s NPs could be reused 14 times with nearly 50% activity remaining, a much higher reusability compared to PLGA-C 1,o NPs and PLGA-C 1,w NPs. These nanovesicles were successfully utilised to generate reduced flavin mononucleotide (FMN) and supply this cofactor to a hydroxylase enzyme that has application for synthesising anti-inflammatory compounds. Therefore, this recycling biocatalyst prepared using the s/o/w method is effective and has the potential for use in combination with other enzymes that require reduced FMN. Application of PLGA-C 1,s NPs may be possible in additional biocatalytic processes for chemical or biochemical production.

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